Western Blot (WB)
(Western blot analysis of GM130 expression in Raji (A), Hela (B) whole cell lysates.)
Rabbit GM130 Polyclonal Antibody | anti-GOLGA2 antibody
Anti-GM130 Antibody
Western Blot (WB)
(Western blot analysis of GM130 expression in Raji (A), Hela (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GM130 expression in Raji (A), Hela (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GM130 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GM130 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]
Uniprot Description
GM130: Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. Part of a larger oligomeric complex. Interacts with p115. Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1. Belongs to the GOLGA2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: cis-Golgi network; ER to Golgi transport vesicle; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi cis cisterna; Golgi membrane; microtubule; nucleus; spindle pole
Molecular Function: microtubule binding; protein binding; protein kinase binding; syntaxin binding
Biological Process: anaphase-promoting complex activation during mitotic cell cycle; asymmetric cell division; centrosome organization and biogenesis; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; microtubule nucleation; negative regulation of autophagy; negative regulation of protein binding; positive regulation of protein amino acid glycosylation; protein amino acid glycosylation; protein homotetramerization; spindle assembly
Research Articles on GOLGA2
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Product Notes
The GOLGA2 golga2 (Catalog #AAA8245771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GM130 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GM130 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the GOLGA2 golga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GM130, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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