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Western Blot (WB) (GOLGA2 polyclonal antibody. Western Blot analysis of GOLGA2 expression in human stomach.)

Mouse anti-Human GOLGA2 Polyclonal Antibody | anti-GOLGA2 antibody

GOLGA2 (Golgin Subfamily A Member 2, 130 kDa cis-Golgi Matrix Protein, GM130 Autoantigen, Golgin-95, GM130, MGC20672)

Gene Names
GOLGA2; GM130
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
GOLGA2; Polyclonal Antibody; GOLGA2 (Golgin Subfamily A Member 2; 130 kDa cis-Golgi Matrix Protein; GM130 Autoantigen; Golgin-95; GM130; MGC20672); Anti -GOLGA2 (Golgin Subfamily A Member 2; anti-GOLGA2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GOLGA2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MQNDRTTISRALSQNRELKEQLAELQSGFVKLTNENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLTSEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDGLDREEEEDEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQSVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERPGRVLPVTTPLHSRSCSCFVRCRTPGSAQAWAATPAFLFFTGLTRMMR
Applicable Applications for anti-GOLGA2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Immunogen
Full length human GOLGA2, aa1-345 (AAH06381.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GOLGA2 polyclonal antibody. Western Blot analysis of GOLGA2 expression in human stomach.)

Western Blot (WB) (GOLGA2 polyclonal antibody. Western Blot analysis of GOLGA2 expression in human stomach.)

Western Blot (WB)

(Western Blot analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane 1: GOLGA2 transfected lysate (37.95kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of GOLGA2 expression in transfected 293T cell line by GOLGA2 polyclonal antibody. Lane 1: GOLGA2 transfected lysate (37.95kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of purified antibody to GOLGA2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of purified antibody to GOLGA2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to GOLGA2 on HeLa cell. [antibody concentration 10ug/ml].)

Immunofluorescence (IF) (Immunofluorescence of purified antibody to GOLGA2 on HeLa cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-GOLGA2 antibody
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
Product Categories/Family for anti-GOLGA2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
113,086 Da
NCBI Official Full Name
Golgin subfamily A member 2
NCBI Official Synonym Full Names
golgin A2
NCBI Official Symbol
GOLGA2
NCBI Official Synonym Symbols
GM130
NCBI Protein Information
Golgin subfamily A member 2; golgin-95; SY11 protein; GM130 autoantigen; Golgi matrix protein GM130; 130 kDa cis-Golgi matrix protein; golgi autoantigen, golgin subfamily a, 2
UniProt Protein Name
Golgin subfamily A member 2
Protein Family
UniProt Gene Name
GOLGA2
UniProt Synonym Gene Names
GM130
UniProt Entry Name
GOGA2_HUMAN

NCBI Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]

Uniprot Description

GM130: Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. Part of a larger oligomeric complex. Interacts with p115. Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65 and ZFPL1. Belongs to the GOLGA2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: Golgi membrane; Golgi apparatus; cis-Golgi network; nucleus; ER to Golgi transport vesicle

Molecular Function: protein binding; syntaxin binding; protein kinase binding

Biological Process: mitotic cell cycle; negative regulation of protein binding

Research Articles on GOLGA2

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Product Notes

The GOLGA2 golga2 (Catalog #AAA6001522) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GOLGA2 (Golgin Subfamily A Member 2, 130 kDa cis-Golgi Matrix Protein, GM130 Autoantigen, Golgin-95, GM130, MGC20672) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOLGA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the GOLGA2 golga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MQNDRTTISR ALSQNRELKE QLAELQSGFV KLTNENMEIT SALQSEQHVK RELGKKLGEL QEKLSELKET VELKSQEAQS LQQQRDQYLG HLQQYVAAYQ QLTSEKEVLH NQLLLQTQLV DQLQQQEAQG KAVAEMARQE LQETQERLEA ATQQNQQLRA QLSLMAHPGE GDGLDREEEE DEEEEEEAVA VPQPMPSIPE DLESREAMVA FFNSAVASAE EEQARLRGQL KEQSVRCRRL AHLLASAQKE PEAAAPAPGT GGDSVCGETH RALQGAMEKL QSRFMELMQE KADLKERPGR VLPVTTPLHS RSCSCFVRCR TPGSAQAWAA TPAFLFFTGL TRMMR. It is sometimes possible for the material contained within the vial of "GOLGA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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