Western Blot (WB)
(Western Blot)
Rabbit anti-Rat Glypican 5 (GPC5) Polyclonal Antibody | anti-GPC5 antibody
Polyclonal Antibody to Glypican 5 (GPC5)
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Glypican 5 (GPC5); Polyclonal Antibody; Polyclonal Antibody to Glypican 5 (GPC5); Glypican Proteoglycan 5; Secreted glypican-5; anti-GPC5 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GPC5. It has been selected for its ability to recognize GPC5 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence Length
524
Applicable Applications for anti-GPC5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:250-2500
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
IHC: 5-20ug/mL; 1:25-100
ICC: 5-20ug/mL; 1:25-100
Cross Reactivity
Rat
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot)
Western Blot (WB)
(Western Blot)
Western Blot (WB)
(Western Blot; Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat GPC5 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat GPC5 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue)
Product Categories/Family for anti-GPC5 antibody
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]
Research Articles on GPC5
1. miR-301b represses the proliferation and invasion of glioma cells by up-regulating GPC5 expression
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Product Notes
The GPC5 (Catalog #AAA2112330) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glypican 5 (GPC5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glypican 5 (GPC5) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:250-2500 IHC: 5-20ug/mL; 1:25-100 ICC: 5-20ug/mL; 1:25-100. Researchers should empirically determine the suitability of the GPC5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glypican 5 (GPC5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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