Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Glypican 4 (GPC4) Polyclonal Antibody | anti-GPC4 antibody
Polyclonal Antibody to Glypican 4 (GPC4)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glypican 4 (GPC4); Polyclonal Antibody; Polyclonal Antibody to Glypican 4 (GPC4); anti-GPC4 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-AQGLAVARD VVNRVSVVNP TAQCTQALLK MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDLEWNN FIDAMLMVAE RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSTVCND ERMAAGNENE DDCWNGKSKS RYLFAVTGNG LANQGNNPEV QVDTSKPDIL ILRQIMAL
MGHHHHHHSG SEF-AQGLAVARD VVNRVSVVNP TAQCTQALLK MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDLEWNN FIDAMLMVAE RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSTVCND ERMAAGNENE DDCWNGKSKS RYLFAVTGNG LANQGNNPEV QVDTSKPDIL ILRQIMAL
Sequence Length
556
Applicable Applications for anti-GPC4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GPC4 (Ala222~Leu468) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2050366
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,562 Da
NCBI Official Full Name
glypican-4
NCBI Official Synonym Full Names
glypican 4
NCBI Official Symbol
Gpc4
NCBI Protein Information
glypican-4
UniProt Protein Name
Glypican 4
Protein Family
UniProt Gene Name
Gpc4
Uniprot Description
GPC4: Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system. Belongs to the glypican family.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq36
Cellular Component: external side of plasma membrane; extracellular space; nucleus; proteinaceous extracellular matrix
Molecular Function: heparan sulfate proteoglycan binding
Research Articles on GPC4
Similar Products
Product Notes
The GPC4 gpc4 (Catalog #AAA2005269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glypican 4 (GPC4) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glypican 4 (GPC4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GPC4 gpc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-AQGLAV ARD VVNRVSVVNP TAQCTQALLK MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDLEWNN FIDAMLMVAE RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA FSARFRPYHP EQRPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSTVCND ERMAAGNENE DDCWNGKSKS RYLFAVTGNG LANQGNNPEV QVDTSKPDIL ILRQIMAL. It is sometimes possible for the material contained within the vial of "Glypican 4 (GPC4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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