Immunohistochemistry (IHC)
(Immunohistochemical staining using GPC3 antibody on human hepatocellular carcinoma tissue. Antibody used at 10 ug/ml. Secondary antibody, HRP conjugated mouse anti-sheep , used at 1:20 dilution. Sections heated for 10 minutes with 10 mM sodium citrate.)
Sheep anti-Human Glypican-3 (GPC3) Polyclonal Antibody | anti-GPC3 antibody
Glypican-3 (GPC3)
Immunohistochemistry (IHC)
(Immunohistochemical staining using GPC3 antibody on human hepatocellular carcinoma tissue. Antibody used at 10 ug/ml. Secondary antibody, HRP conjugated mouse anti-sheep , used at 1:20 dilution. Sections heated for 10 minutes with 10 mM sodium citrate.)
Immunohistochemistry (IHC)
(Immunohistochemical staining using GPC3 antibody on human hepatocellular carcinoma tissue. Antibody used at 10 ug/ml. Secondary antibody, HRP conjugated mouse anti-sheep , used at 1:20 dilution. Sections heated for 10 minutes with 10 mM sodium citrate.)
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
Uniprot Description
Function: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Ref.8
Subunit structure: Interacts with DPP4. Ref.8
Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side
By similarity. Secreted glypican-3: Secreted › extracellular space
By similarity.
Tissue specificity: Highly expressed in lung, liver and kidney.
Involvement in disease: Simpson-Golabi-Behmel syndrome 1 (SGBS1) [MIM:312870]: A condition characterized by pre- and postnatal overgrowth (gigantism), facial dysmorphism and a variety of inconstant visceral and skeletal malformations. Characteristic dysmorphic features include macrocephaly with coarse, distinctive facies with a large protruding jaw, broad nasal bridge and cleft palate. Cardiac defects are frequent.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.10
Sequence similarities: Belongs to the glypican family.
Research Articles on GPC3
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Product Notes
The GPC3 gpc3 (Catalog #AAA395497) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Glypican-3 (GPC3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glypican-3 (GPC3) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). Researchers should empirically determine the suitability of the GPC3 gpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glypican-3 (GPC3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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