Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Rabbit Glypican 3 Polyclonal Antibody | anti-GPC3 antibody
Polyclonal Antibody to Glypican 3 (GPC3)
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GPC3, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GPC3, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Esophagus Tissue;Primary Ab: 30ug/ml Rabbit Anti-MouseGPC3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Mouse Esophagus Tissue;Primary Ab: 30ug/ml Rabbit Anti-MouseGPC3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue)
NCBI and Uniprot Product Information
Uniprot Description
GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: X|X A5
Cellular Component: anchored to plasma membrane; extracellular region; integral component of plasma membrane; lysosome; membrane; plasma membrane; proteinaceous extracellular matrix
Molecular Function: heparan sulfate proteoglycan binding; peptidase inhibitor activity; protein binding
Biological Process: anterior/posterior axis specification; body morphogenesis; bone mineralization; branching involved in ureteric bud morphogenesis; embryonic hindlimb morphogenesis; kidney development; lung development; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of growth; negative regulation of peptidase activity; negative regulation of smoothened signaling pathway; organ morphogenesis; osteoclast differentiation; positive regulation of BMP signaling pathway; positive regulation of endocytosis; positive regulation of glucose import; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; regulation of growth
Research Articles on GPC3
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Product Notes
The GPC3 gpc3 (Catalog #AAA2027574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glypican 3 (GPC3) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Glypican 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GPC3 gpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glypican 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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