Rabbit anti-Human Glycogen Synthase Kinase 3 beta Polyclonal Antibody | anti-GSK3B antibody

Glycogen Synthase Kinase 3 beta, phosphorylated (Ser9) (GSK3b)

Cat. Num. AAA630608

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Glycogen Synthase Kinase 3 beta; Polyclonal Antibody; phosphorylated (Ser9) (GSK3b); Anti -Glycogen Synthase Kinase 3 beta; anti-GSK3B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human phosphorylated human GSK3B, pS9. Assay by IEP resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated GSK3B.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as liquid in PBS, pH7.2, 0.01% sodium azide.

• Applicable Applications for anti-GSK3B antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.; Dilution: ELISA: 1:10,000-1:50,000; Western Blot: 1:1500-1:3000.; Immunohistochemistry: 1:200-1:1000; Immunofluorescence: 1:200-1:1000.
• Immunogen: A synthetic peptide, RPRTTSFAE, corresponding to the aa 4-12 of human GSK3 beta.
• Positive Control: Serum starved 293T whole cell lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GSK3B antibody

Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation. GSK 3B participates in the Wnt signaling pathway and has been implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. GSK 3B phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.

Product Categories/Family for anti-GSK3B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5730335
• NCBI GeneID: 2932
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89; Q6FI27
• Molecular Weight: 46,744 Da
• NCBI Official Full Name: glycogen synthase kinase 3 beta
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; OTTHUMP00000165239; OTTHUMP00000165240
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: GSK3B
• UniProt Entry Name: GSK3B_HUMAN

NCBI Description

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.26; EC 2.7.11.1; Kinase, protein; CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 3q13.3

Cellular Component: nucleoplasm; centrosome; growth cone; cell soma; perinuclear region of cytoplasm; cytoplasm; dendritic spine; plasma membrane; dendritic shaft; beta-catenin destruction complex; ribonucleoprotein complex; cytosol; nucleus; lipid raft

Molecular Function: p53 binding; beta-catenin binding; protein kinase binding; integrin binding; protein serine/threonine kinase activity; NF-kappaB binding; ionotropic glutamate receptor binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: circadian rhythm; glycogen metabolic process; fat cell differentiation; negative regulation of MAP kinase activity; axon guidance; genetic imprinting; re-entry into mitotic cell cycle; positive regulation of protein binding; nerve growth factor receptor signaling pathway; positive regulation of axon extension; response to lithium ion; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; response to drug; regulation of microtubule-based process; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hippocampus development; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; peptidyl-serine phosphorylation; negative regulation of neuron maturation; organ morphogenesis; positive regulation of protein complex assembly; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity