Rabbit anti-Mouse Glycogen Synthase Kinase 3 Alpha (GSK3a) Polyclonal Antibody | anti-GSK3a antibody

Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a)

Cat. Num. AAA2016814

• Gene Names: Gsk3a; 2700086H06Rik
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Glycogen Synthase Kinase 3 Alpha (GSK3a); Polyclonal Antibody; Polyclonal Antibody to Glycogen Synthase Kinase 3 Alpha (GSK3a); anti-GSK3a antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against GSK3a. It has been selected for its ability to recognize GSK3a in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLITPIIY IKVYMYQLFR SLAYIHSQGV CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS SKTPPEAIAL
• Sequence Length: 483

• Applicable Applications for anti-GSK3a antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant GSK3a (Ile122~Glu406) expressed in E.coli.
• Cross Reactivity: Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2045250
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSK3a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSK3a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSK3a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB)

(Western Blot: Sample: Mouse Small intestine lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GSK3a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Ovary Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 72384361
• NCBI GeneID: 606496
• NCBI Accession #: NP_001026837.1
• NCBI GenBank Nucleotide #: NM_001031667.1
• UniProt Accession #: Q2NL51
• Molecular Weight: 51,661 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: Gsk3a
• NCBI Official Synonym Symbols: 2700086H06Rik
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: Gsk3a
• UniProt Synonym Gene Names: GSK-3 alpha

Uniprot Description

GSK3A: a constitutively active proline-directed protein kinase of the GSK family. A negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubule. Phosphorylates and inactivates glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Generally requires primed phosphorylation sites C-terminal to the targeted serine or threonine residues in its substrate. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factor deprivation. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized b-catenin mutants that lack GSK3 phosphorylation sites.

Protein type: CMGC group; EC 2.7.11.1; EC 2.7.11.26; GSK family; GSK subfamily; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7|7 A3

Cellular Component: cytoplasm; microtubule

Molecular Function: ATP binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: aging; carbohydrate metabolic process; cardiac left ventricle morphogenesis; cell migration; cellular response to insulin stimulus; cellular response to lithium ion; cellular response to organic cyclic compound; genetic imprinting; glycogen metabolic process; insulin receptor signaling pathway; negative regulation of dendrite development; negative regulation of glucose import; negative regulation of glycogen synthase activity, transferring glucose-1-phosphate; negative regulation of insulin receptor signaling pathway; negative regulation of signal transduction; negative regulation of TOR signaling; nervous system development; peptidyl-serine phosphorylation; phosphorylation; positive regulation of adrenergic receptor signaling pathway; positive regulation of amyloid-beta formation; positive regulation of cAMP biosynthetic process; positive regulation of glycogen (starch) synthase activity; positive regulation of heart contraction; positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; positive regulation of neuron apoptotic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-threonine phosphorylation; positive regulation of protein targeting to mitochondrion; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure; Wnt signaling pathway