Glycogen Synthase Kinase 3 Alpha (GSK3a) Recombinant Protein | GSK3a recombinant protein

Recombinant Glycogen Synthase Kinase 3 Alpha (GSK3a)

Cat. Num. AAA2018527

• Gene Names: Gsk3a; 2700086H06Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glycogen Synthase Kinase 3 Alpha (GSK3a); Recombinant Glycogen Synthase Kinase 3 Alpha (GSK3a); GSK3a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLITPIIY IKVYMYQLFR SLAYIHSQGV CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK AHPWTKVFKS SKTPPEAIALCSSLLEYTPS SRLSPLEACA HSFFDE
• Sequence Length: 490

• Applicable Applications for GSK3a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ile122~Glu406 (Accession # Q2NL51) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 72384361
• NCBI GeneID: 606496
• NCBI Accession #: NP_001026837.1
• NCBI GenBank Nucleotide #: NM_001031667.1
• UniProt Accession #: Q2NL51
• Molecular Weight: 36.5kDa
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: Gsk3a
• NCBI Official Synonym Symbols: 2700086H06Rik
• NCBI Protein Information: glycogen synthase kinase-3 alpha; GSK-3 alpha; serine/threonine-protein kinase GSK3A
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• Protein Family: Glycogen synthase kinase
• UniProt Gene Name: Gsk3a
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_MOUSE

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.26; EC 2.7.11.1; CMGC group; GSK family; GSK subfamily

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: glycogen metabolic process; proteasomal ubiquitin-dependent protein catabolic process; nervous system development; negative regulation of TOR signaling pathway; cell migration; genetic imprinting; Wnt receptor signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of signal transduction; negative regulation of glucose import; negative regulation of transferase activity; cellular response to insulin stimulus; positive regulation of cAMP biosynthetic process; carbohydrate metabolic process; insulin receptor signaling pathway; regulation of systemic arterial blood pressure; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction; phosphorylation