Rabbit anti-Human Glycogen Phosphorylase Polyclonal Antibody | anti-PYGM antibody

Glycogen Phosphorylase, Muscle Form, CT (PYGM, Myophosphorylase) (PE)

Cat. Num. AAA6478494

• Gene Names: PYGM; GSD5
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Glycogen Phosphorylase; Polyclonal Antibody; Muscle Form; CT (PYGM; Myophosphorylase) (PE); anti-PYGM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PYGM.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 842

• Applicable Applications for anti-PYGM antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PYGM (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PYGM antibody

Antibodies; Enzymes; Phosphorylase

References

1. Tsoi, S.C., et al., J. Soc. Gynecol. Investig. 10(8):496-502 (2003). 2. Bruno, C., et al., Neuromuscul. Disord. 12(5):498-500 (2002). 3. Hadjigeorgiou, G.M., et al., Neuromuscul. Disord. 12(9):824-827 (2002). 4. Deschauer, M., et al., Mol. Genet. Metab. 74(4):489-491 (2001). 5. Kubisch, C., et al., Hum. Mutat. 12(1):27-32 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 5032009
• NCBI GeneID: 5837
• NCBI Accession #: NP_005600
• NCBI GenBank Nucleotide #: NP_005600.1
• UniProt Accession #: P11217
• Molecular Weight: 97.092
• NCBI Official Full Name: glycogen phosphorylase, muscle form isoform 1
• NCBI Official Synonym Full Names: glycogen phosphorylase, muscle associated
• NCBI Official Symbol: PYGM
• NCBI Official Synonym Symbols: GSD5
• NCBI Protein Information: glycogen phosphorylase, muscle form
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGM
• UniProt Entry Name: PYGM_HUMAN

NCBI Description

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Phosphorylase; Carbohydrate Metabolism - starch and sucrose; Transferase; Endoplasmic reticulum; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytosol

Molecular Function: glycogen phosphorylase activity; nucleotide binding; pyridoxal phosphate binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Glycogen Storage Disease V