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Testing Data (Peptide-+)

anti-Human Collagen V alpha1 Antibody | anti-COL5A1 antibody

Collagen V alpha1 Antibody

Gene Names
COL5A1; EDSC; EDSCL1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Collagen V alpha1; Collagen V alpha1 Antibody; Collagen alpha-1(V) chain [Precursor]; anti-COL5A1 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Collagen V alpha1 antibody detects endogenous levels of total Collagen V alpha1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1838
Applicable Applications for anti-COL5A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Collagen V alpha1.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human heart tissue using Collagen V alpha1 antibody. Western blot analysis of extracts from HeLa cells, using Collagen V alpha1 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using Collagen V alpha1 antibody. Western blot analysis of extracts from HeLa cells, using Collagen V alpha1 antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 antibody.)

Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 antibody.)
References
Takahara K., J. Biol. Chem. 266:13124-13129(1991). Greenspan D.S., J. Biol. Chem. 266:24727-24733(1991). Humphray S.J., Nature 429:369-374(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
183,560 Da
NCBI Official Full Name
collagen alpha-1(V) chain isoform 1 preproprotein
NCBI Official Synonym Full Names
collagen type V alpha 1 chain
NCBI Official Symbol
COL5A1
NCBI Official Synonym Symbols
EDSC; EDSCL1
NCBI Protein Information
collagen alpha-1(V) chain
UniProt Protein Name
Collagen alpha-1(V) chain
Protein Family
UniProt Gene Name
COL5A1

NCBI Description

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.

Research Articles on COL5A1

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Product Notes

The COL5A1 col5a1 (Catalog #AAA852751) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Collagen V alpha1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen V alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the COL5A1 col5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen V alpha1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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