Immunohistochemistry (IHC)
(IHC Analysis: Representative staining pattern on formalin-fixed, paraffin-embedded mouse spinal cord. Tissue was pretreated with citrate, pH 6.0 using antigen retrieval techniques. MBS621830 diluted to 1:100-500.)
Rabbit anti-Human, Mouse Glycine Receptor alpha 3 subunit Polyclonal Antibody | anti-GLRA3 antibody
Glycine Receptor alpha 3 subunit (GLRA3, ligand gated ion channel, glycine receptor, alpha-3 polypeptide, glycine receptor, alpha 3) (MaxLight 490)
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
Glycine Receptor alpha 3 subunit; Polyclonal Antibody; Glycine Receptor alpha 3 subunit (GLRA3; ligand gated ion channel; glycine receptor; alpha-3 polypeptide; alpha 3) (MaxLight 490); anti-GLRA3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Glycine Receptor a3 subunit at ~48kD. Species Crossreactivity: mouse. Species Sequence Homology: rat, 92%
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-GLRA3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:500 detected Glycine Receptor alpha 3 subunit in mouse spinal cord and mouse eye tissue.
WB: 0.1ug/ml detected glycine receptor alpha 3 subunit on 10ug of fetal human brain tissue lysate.
Applications are based on unconjugated antibody.
WB: 0.1ug/ml detected glycine receptor alpha 3 subunit on 10ug of fetal human brain tissue lysate.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide from the C-terminus of human GLRA3, conjugated to KLH (Accession #NP_006520.2). Cellular Localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Conjugate
MaxLight490
Positive Control
Fetal human brain tissue lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(IHC Analysis: Representative staining pattern on formalin-fixed, paraffin-embedded mouse spinal cord. Tissue was pretreated with citrate, pH 6.0 using antigen retrieval techniques. MBS621830 diluted to 1:100-500.)
Immunohistochemistry (IHC)
(IHC Analysis: Representative staining pattern on formalin-fixed, paraffin-embedded mouse spinal cord. Tissue was pretreated with citrate, pH 6.0 using antigen retrieval techniques. MBS621830 diluted to 1:100-500.)
Immunohistochemistry (IHC)
(IHC Analysis: Representative staining pattern on formalin-fixed, paraffin-embedded mouse eye. Tissue pretreated with citrate, pH 6.0 using antigen retrieval techniques. MBS621830 diluted to 1:100-1:300.)
Immunohistochemistry (IHC)
(IHC Analysis: Representative staining pattern on formalin-fixed, paraffin-embedded mouse eye. Tissue pretreated with citrate, pH 6.0 using antigen retrieval techniques. MBS621830 diluted to 1:100-1:300.)
Western Blot (WB)
(Western Blot Analysis: Fetal human brain lysate was resolved by electrophoresis, transferred to PVDF and probed withMBS621830. Proteins were visualized using IgG (HRP) Pab donkey x rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates Glycine Receptor a3 subunit (48kD).)
Western Blot (WB)
(Western Blot Analysis: Fetal human brain lysate was resolved by electrophoresis, transferred to PVDF and probed withMBS621830. Proteins were visualized using IgG (HRP) Pab donkey x rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates Glycine Receptor a3 subunit (48kD).)
Product Categories/Family for anti-GLRA3 antibody
References
1. Nikolic, Z., et al. (1998). The Journal Of Biological Chemistry. 273(31):19708-19714.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,963 Da
NCBI Official Full Name
glycine receptor subunit alpha-3 isoform a
NCBI Official Synonym Full Names
glycine receptor alpha 3
NCBI Official Symbol
GLRA3
NCBI Protein Information
glycine receptor subunit alpha-3
UniProt Protein Name
Glycine receptor subunit alpha-3
Protein Family
UniProt Gene Name
GLRA3
UniProt Entry Name
GLRA3_HUMAN
NCBI Description
This gene encodes a member of the ligand-gated ion channel protein family. The encoded protein is a member of the glycine receptor subfamily. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Research Articles on GLRA3
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Product Notes
The GLRA3 glra3 (Catalog #AAA6478468) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glycine Receptor alpha 3 subunit (GLRA3, ligand gated ion channel, glycine receptor, alpha-3 polypeptide, glycine receptor, alpha 3) (MaxLight 490) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glycine Receptor alpha 3 subunit can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:500 detected Glycine Receptor alpha 3 subunit in mouse spinal cord and mouse eye tissue. WB: 0.1ug/ml detected glycine receptor alpha 3 subunit on 10ug of fetal human brain tissue lysate. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GLRA3 glra3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glycine Receptor alpha 3 subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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