Western Blot (WB)
(Western blot analysis GON4L using Jurkat whole cell lysates)
Rabbit anti-Human, Rat GON4L Polyclonal Antibody | anti-GON4L antibody
GON4L Antibody
Gene Names
GON4L; GON4; YARP; GON-4
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
GON4L; Polyclonal Antibody; GON4L Antibody; GON 4; GON-4 homolog; GON-4-like protein; GON4; GON4L_HUMAN; YARP; YY1AP related protein; YY1AP related protein1; anti-GON4L antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GON4L antibody detects endogenous levels of total GON4L
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
2241
Applicable Applications for anti-GON4L antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA(peptide) (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
ELISA(peptide) 1:20000 - 1:40000
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Optimal dilutions/concentrations should be determined by the end user.
IHC: 1:200
ELISA(peptide) 1:20000 - 1:40000
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Optimal dilutions/concentrations should be determined by the end user.
Immunogen
A synthesized peptide derived from human GON4L, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis GON4L using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis GON4L using Jurkat whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609854 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609854 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-GON4L antibody
Function: Has transcriptional repressor activity, probably as part of a complex with YY1, SIN3A AND HDAC1. Required for B cell lymphopoiesis.
Subunit Structure: Found in a complex with YY1, SIN3A and HDAC1.
Subunit Structure: Found in a complex with YY1, SIN3A and HDAC1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
249 kDa
NCBI Official Full Name
GON-4-like protein isoform c
NCBI Official Synonym Full Names
gon-4 like
NCBI Official Symbol
GON4L
NCBI Official Synonym Symbols
GON4; YARP; GON-4
NCBI Protein Information
GON-4-like protein
UniProt Protein Name
GON-4-like protein
Protein Family
UniProt Gene Name
GON4L
UniProt Synonym Gene Names
GON4; KIAA1606
Uniprot Description
Has transcriptional repressor activity, probably as part of a complex with YY1, SIN3A AND HDAC1. Required for B cell lymphopoiesis.
Research Articles on GON4L
Similar Products
Product Notes
The GON4L gon4l (Catalog #AAA9609854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GON4L Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GON4L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA(peptide) (EIA). WB: 1:1000-3000 IHC: 1:200 ELISA(peptide) 1:20000 - 1:40000 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Optimal dilutions/concentrations should be determined by the end user. . Researchers should empirically determine the suitability of the GON4L gon4l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GON4L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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