Western Blot (WB)
(Western Blot; Sample: Recombinant GSTa1, Human.)
Rabbit Glutathione S Transferase Alpha 1 (GSTa1) Polyclonal Antibody | anti-GSTa1 antibody
Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1)
MGHHHHHHSGS-AEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF
Immunocytochemistry: 2-20 ug/mL
Immunohistochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant GSTa1, Human.)
Western Blot (WB)
(Western Blot; Sample: Recombinant GSTa1, Human.)
Western Blot (WB)
(Western Blot; Sample: Mouse Liver lysate;Primary Ab: 2ug/ml Rabbit Anti-Human GSTa1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG PolyclonalAntibody (MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Mouse Liver lysate;Primary Ab: 2ug/ml Rabbit Anti-Human GSTa1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG PolyclonalAntibody (MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human GSTa1Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgGPolyclonal Antibody (MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human GSTa1Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgGPolyclonal Antibody (MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
GSTA1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Alpha family.
Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 6p12.2
Cellular Component: cytosol
Molecular Function: glutathione peroxidase activity; glutathione transferase activity
Biological Process: epithelial cell differentiation; glutathione metabolic process; linoleic acid metabolic process; metabolic process
Research Articles on GSTa1
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Product Notes
The GSTa1 gsta1 (Catalog #AAA2004800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S Transferase Alpha 1 (GSTa1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Western Blotting: 0.5-2 ug/mL Immunocytochemistry: 2-20 ug/mL Immunohistochemistry: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GSTa1 gsta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-AEKPKLHY F NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Alpha 1 (GSTa1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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