Western Blot (WB)
(Western Blot: Sample: Human a431 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Glutaminyl tRNA Synthetase Polyclonal Antibody | anti-QARS antibody
Polyclonal Antibody to Glutaminyl tRNA Synthetase (QARS)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human a431 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human a431 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human QARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded stomach tissue))
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Uniprot Description
QARS: catalyzes the formation of L-glutaminyl-tRNA. Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: Aminoacyl-tRNA synthetase; EC 6.1.1.18; Ligase; Mitochondrial; Translation; Translation regulation
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; mitochondrial matrix; protein complex
Molecular Function: ATP binding; glutamine-tRNA ligase activity; ligase activity; protein binding; protein kinase binding; protein kinase inhibitor activity
Biological Process: brain development; glutaminyl-tRNA aminoacylation; negative regulation of apoptotic signaling pathway; negative regulation of protein kinase activity; negative regulation of stress-activated MAPK cascade; negative regulation of transcription, DNA-dependent; tRNA aminoacylation for protein translation
Disease: Microcephaly, Progressive, With Seizures And Cerebral And Cerebellar Atrophy
Research Articles on QARS
Similar Products
Product Notes
The QARS qars (Catalog #AAA2033981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutaminyl tRNA Synthetase (QARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutaminyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the QARS qars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutaminyl tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
