anti-Rat Glutamate Transporter EAAT4 Polyclonal Antibody | anti-SLC1A6 antibody

Anti - Glutamate Transporter EAAT4 (GTRAP41, Excitatory Amino Acid Transporter 4)

Cat. Num. AAA614813

• Gene Names: SLC1A6; EAAT4
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Glutamate Transporter EAAT4; Polyclonal Antibody; Anti - Glutamate Transporter EAAT4 (GTRAP41; Excitatory Amino Acid Transporter 4); Glutamate Transporter EAAT4 (GTRAP41; anti-SLC1A6 antibody

• Host: Host: Rabbit; Source: Rat
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The rat peptide is 86% conserved in human GTRAP41/beta-spectrin 3. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
• Sequence Length: 564

• Applicable Applications for anti-SLC1A6 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. GTRAP41 is ~270kD protein (1).; ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: A 15 AA synthetic peptide near the C-terminus of rat GTRAP41 was synthesized, conjugated to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC1A6 antibody

Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently two proteins, designated GTRAP41 and GTRAP48 (for glutamate transporter EAAT4 associated protein) that specifically interact with the intracellular carboxy-terminal domain of EAAT4 have been cloned and characterized. The proteins are proposed to modulate glutamate transport activity. GTRAP41 is 2388 aa protein (~270kD). It has seventeen 16-amino acid spectrin repeats, 2 alpha-actinin domains, and a pleckstrin homology domain. GTRAP48 is 1527 aa (~170kD). GTRAP48 possesses a PDZ domain, a regulatory G protein-signaling sequence, tandem dbl homology and pleckstrin homology domains characteristic of guanine nucleotide exchange factors for the Rho family of G proteins, and 2 proline-rich sequences. brain tissue. GTRAP41 and GTRAP48 are mainly expressed in the brain, and lower levels were also detected in liver and kidney. EAAT4, GTRAP41, and GTRAP48 are all immunolocalized in cerebellar Purkinje cell soma and dendrites, with little axonal staining. GTRAP48 was found to activate Rho. Expression of GTRAP48 induced the reorganization of the actin cytoskeleton. When GTRAP41 and GTRAP48 are coexpressed, GTRAP41 colocalized with actin in structures that resemble actin-stress fibers, a typical Rho-dependent effect. Overexpression of GTRAP41 and GTRAP48 enhanced glutamate uptake. GTRAP41 and GTRAP48 may therefore enhance glutamate transport either through an increase in the catalytic rate of the transporter or through an increase in cell-surface availability or by stabilizing EAAT4 at the membrane.

Product Categories/Family for anti-SLC1A6 antibody

Abs to Proteins

References

• 1. Jackson M et al (2001) Nature 410, 89-93; Ohara O et al (1998) Brain Res. Mol. Brain res. 57, 181; Stanekewich MC et al (1998) PNAS 95, 14158; Rumenapp U eat al (1999) FEBS Lett. 459, 313; Fukuhara JBC 274, 5868-5879

NCBI and Uniprot Product Information

• NCBI GI #: 6978311
• NCBI GeneID: 403749
• UniProt Accession #: Q9N1R2
• Molecular Weight: 61,466 Da
• NCBI Official Full Name: glutamate transporter
• NCBI Official Symbol: SLC1A6
• NCBI Official Synonym Symbols: EAAT4
• NCBI Protein Information: excitatory amino acid transporter 4; glutamate transporter; solute carrier family 1 member 6; sodium-dependent glutamate/aspartate transporter
• UniProt Protein Name: Excitatory amino acid transporter 4
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: SLC1A6
• UniProt Synonym Gene Names: EAAT4
• UniProt Entry Name: EAA4_CANFA