Rabbit anti-Human ZEB1 Polyclonal Antibody | anti-ZEB1 antibody

ZEB1 Antibody

Cat. Num. AAA151570

• Gene Names: ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ZEB1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ZEB1; Polyclonal Antibody; ZEB1 Antibody; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; TCF-8; zinc finger E-box binding homeobox 1; anti-ZEB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of ZEB1 are known to exist.
• Purity/Purification: ZEB1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1,108

• Applicable Applications for anti-ZEB1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.
• Buffer: ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ZEB1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 μg/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 μg/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 μg/ml.)

Related Product Information for anti-ZEB1 antibody

ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.

NCBI and Uniprot Product Information

• NCBI GI #: 55957123
• NCBI GeneID: 6935
• UniProt Accession #: P37275; Q12924; Q13800; Q2KJ05; Q5T968; Q5VZ84; Q8NB68; B4DJV0; B4DUW9; E9PCM7; F5H4I8
• Molecular Weight: Predicted: 124 kDa; ; Observed: 130 kDa
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 1
• NCBI Official Symbol: ZEB1
• NCBI Official Synonym Symbols: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• NCBI Protein Information: zinc finger E-box-binding homeobox 1
• UniProt Protein Name: Zinc finger E-box-binding homeobox 1
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB1
• UniProt Synonym Gene Names: AREB6; TCF8; TCF-8
• UniProt Entry Name: ZEB1_HUMAN

NCBI Description

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Uniprot Description

TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: C2H2-type zinc finger protein; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; forebrain development; regulation of smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; immune response; embryonic camera-type eye morphogenesis; response to activity; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation

Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6