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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human Glutamate Receptor Interacting Protein 1 (GRIP1) Polyclonal Antibody | anti-GRIP1 antibody

Polyclonal Antibody to Glutamate Receptor Interacting Protein 1 (GRIP1)

Gene Names
GRIP1; GRIP; FRASRS3
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glutamate Receptor Interacting Protein 1 (GRIP1); Polyclonal Antibody; Polyclonal Antibody to Glutamate Receptor Interacting Protein 1 (GRIP1); anti-GRIP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GRIP1. It has been selected for its ability to recognize GRIP1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-GFAGAAD SAETEQEENF WSQALEDLET CGQSGILREL EEKADRRVSL RNMTLLATIM SGSTMSLNHE APTPRSQLGR QASFQERSSS RPHYSQTTRS NTLPSDVGRK SVTLRKMKQE IKEIMSPTPV ELHKVTLYKD SDMEDFGFSV ADGLLEKGVY VKNIRPAGPG DLGGLKPYDR LLQVNHVRTR DFDCCLVVPL IAE
Sequence Length
1076
Applicable Applications for anti-GRIP1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GRIP1 (Gly874~Glu1073) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058650
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
116,371 Da
NCBI Official Full Name
glutamate receptor-interacting protein 1 isoform 2
NCBI Official Synonym Full Names
glutamate receptor interacting protein 1
NCBI Official Symbol
GRIP1
NCBI Official Synonym Symbols
GRIP; FRASRS3
NCBI Protein Information
glutamate receptor-interacting protein 1
UniProt Protein Name
Glutamate receptor-interacting protein 1
UniProt Gene Name
GRIP1
UniProt Synonym Gene Names
GRIP-1

NCBI Description

This gene encodes a member of the glutamate receptor interacting protein family. The encoded scaffold protein binds to and mediates the trafficking and membrane organization of a number of transmembrane proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]

Uniprot Description

GRIP1: a scaffold protein for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific locations in neurons. Interacts with EphA7, EphB2, KIF5A, -B, -C, GluR2, -3, GRIPAP1, liprin A1, PPFIA4, FRAS1, and PTPRF.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cell junction; cytosol; dendrite; endoplasmic reticulum; membrane raft; neuron projection; plasma membrane; postsynaptic membrane; recycling endosome

Molecular Function: androgen receptor binding; beta-catenin binding; glucocorticoid receptor binding; protein binding; protein C-terminus binding; receptor signaling complex scaffold activity; transcription coactivator activity

Biological Process: androgen receptor signaling pathway; dendrite development; positive regulation of transcription, DNA-templated; protein localization

Disease: Fraser Syndrome; Fraser Syndrome 3

Research Articles on GRIP1

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Product Notes

The GRIP1 grip1 (Catalog #AAA2003139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamate Receptor Interacting Protein 1 (GRIP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Receptor Interacting Protein 1 (GRIP1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GRIP1 grip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-GFAGA AD SAETEQEENF WSQALEDLET CGQSGILREL EEKADRRVSL RNMTLLATIM SGSTMSLNHE APTPRSQLGR QASFQERSSS RPHYSQTTRS NTLPSDVGRK SVTLRKMKQE IKEIMSPTPV ELHKVTLYKD SDMEDFGFSV ADGLLEKGVY VKNIRPAGPG DLGGLKPYDR LLQVNHVRTR DFDCCLVVPL IAE. It is sometimes possible for the material contained within the vial of "Glutamate Receptor Interacting Protein 1 (GRIP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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