Rabbit anti-Human ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

ZAP70 Polyclonal Antibody

Cat. Num. AAA3013707

• Gene Names: ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: ZAP70; Polyclonal Antibody; ZAP70 Polyclonal Antibody; SRK; 70 kDa zeta associated protein; 70 kDa zeta-associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; Selective T cell defect; STD; Syk related tyrosine kinase; Syk-related tyrosine kinase; Truncated ZAP kinase; Tyrosine protein kinase ZAP70; Tyrosine-protein kinase ZAP-70; TZK; ZAP 70; Zeta chain associated protein kinase 70kD; Zeta chain associated protein kinase 70kDa; Zeta chain associated protein kinase 70kDa isoform 1; Zeta chain associated protein kinase 70kDa isoform 2; Zeta chain of T cell receptor associated protein kinase 70; Zeta chain TCR associated protein kinase 70kD; Zeta chain TCR associated protein kinase 70kDa; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZAP70 polyclonal antibody detects endogenous levels of ZAP70 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 619

• Applicable Applications for anti-ZAP70 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100; IP: 1:10-1:50
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.)

Immunocytochemistry (ICC)

(ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-ZAP70 antibody

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and e chains and a zeta-containing homodimer or heterodimer. The disulfide-linked Ti alpha-beta heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti alpha and beta are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and zeta subunits suggest a role in signal transduction. Of these, the zeta chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NP_001070.2
• UniProt Accession #: P43403
• Molecular Weight: 70kDa
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta chain of T cell receptor associated protein kinase 70
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: cell migration; adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of T cell differentiation; innate immune response; immune response; positive regulation of alpha-beta T cell proliferation; beta selection; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; positive thymic T cell selection

Disease: Selective T-cell Defect