Rabbit GluR1 Polyclonal Antibody | anti-Gria1 antibody

RABBIT ANTI RAT GLUTAMATE RECEPTOR 1 (C-TERMINAL)

Cat. Num. AAA220876

• Gene Names: Gria1; GluA1; gluR-A
• Reactivity: Hamster, Human, Monkey
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: GluR1; Polyclonal Antibody; RABBIT ANTI RAT GLUTAMATE RECEPTOR 1 (C-TERMINAL); anti-Gria1 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.
• Form/Format: Purified; Purified Ig - liquid
• Concentration: Ig concentration 1 mg/ml (varies by lot)
• Sequence: S-H-S-S-G-M-P-L-G-A-T-G-L
• Sequence Length: 907

• Applicable Applications for anti-Gria1 antibody: Immunohistology Frozen, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells.; Immunohistology: Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.; Immunohistology - Frozen: Minimum Dilution: 1 ug/ml; Maximum Dilution: 3 ug/ml; Western Blotting: Minimum Dilution: 0.5 ug/ml; Maximum Dilution: 2.5 ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); Preparation
• Immunogen: Synthetic peptide corresponding to the C terminal region of the native molecule conjugated to Bovine Serum Albumin.; Buffer Solution
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-Gria1 antibody

MBS220876 recognises the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells.

NCBI and Uniprot Product Information

• NCBI GI #: 29789269
• NCBI GeneID: 50592
• NCBI Accession #: NP_113796.1
• NCBI GenBank Nucleotide #: NM_031608.1
• UniProt Accession #: P19490
• Molecular Weight: 101,555 Da
• NCBI Official Full Name: glutamate receptor 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA 1
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: GluA1; gluR-A
• NCBI Protein Information: glutamate receptor 1; AMPA-selective glutamate receptor 1; GluR1; gluR-1; gluR-K1; glutamate receptor A; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1)
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_RAT

NCBI Description

mouse homolog may play a role in regulation of neuronal synaptic plasticity and conditioned reinforcement learning [RGD, Feb 2006]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated; Channel, calcium

Cellular Component: endoplasmic reticulum membrane; neuron projection; cell surface; protein complex; endoplasmic reticulum; dendrite; early endosome; postsynaptic density; dendritic spine; integral to membrane; excitatory synapse; cytosol; asymmetric synapse; postsynaptic membrane; recycling endosome; synaptic vesicle; membrane; cell soma; plasma membrane; ionotropic glutamate receptor complex; synapse; dendritic shaft; cell junction; neuromuscular junction

Molecular Function: identical protein binding; protein domain specific binding; protein homodimerization activity; myosin V binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; protein kinase binding; PDZ domain binding; small GTPase binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; beta-2 adrenergic receptor binding; protein kinase A binding; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; adenylate cyclase binding

Biological Process: regulation of synaptic transmission; response to drug; synaptic transmission, glutamatergic; response to peptide hormone stimulus; response to toxin; response to arsenic; response to lithium ion; generation of action potential; regulation of synaptic plasticity; response to cocaine; response to organic cyclic substance; positive regulation of synaptic transmission; response to estradiol stimulus; synaptic transmission; long-term memory; ionotropic glutamate receptor signaling pathway; spinal cord development; receptor internalization; regulation of receptor recycling; response to electrical stimulus; positive regulation of membrane potential