Typical Testing Data/Standard Curve (for reference only)
Mouse CD5L/CT 2 ELISA Kit | CD5L elisa kit
Mouse CD5L/CT 2 PicoKine Fast ELISA Kit
Gene Names
Cd5l; 1/6; CT2; Pdp; Api6; mAIM; AAC-11; AI047839; Sp-alpha
Reactivity
Mouse
Synonyms
CD5L/CT 2; Mouse CD5L/CT 2 PicoKine Fast ELISA Kit; mouse CD5L; CD5 antigen-like; Apoptosis inhibitor expressed by macrophages; mAIM; Apoptosis inhibitory 6; SP-alpha; Cd5l; Aim; Api6; CD5L elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Cd5l
Sequence Length
352
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD5L elisa kit
Principle of the Assay: The Picokine™ Mouse Cd5l Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Cd5l with a 96-well strip plate that is pre-coated with antibody specific for Cd5l. The detection antibody is a biotinylated antibody specific for Cd5l. The capture antibody is polyclonal antibody from goat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Cd5l with immunogen: Expression system for standard: NSO; Immunogen sequence: E22-V352. The kit is analytically validated with ready to use reagents. To measure Mouse Cd5l, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Cd5l in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Cd5l in the sample.!!Background/Introduction: CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD5 antigen-like
NCBI Official Synonym Full Names
CD5 antigen-like
NCBI Official Symbol
Cd5l
NCBI Official Synonym Symbols
1/6; CT2; Pdp; Api6; mAIM; AAC-11; AI047839; Sp-alpha
NCBI Protein Information
CD5 antigen-like
UniProt Protein Name
CD5 antigen-like
Protein Family
UniProt Gene Name
Cd5l
UniProt Synonym Gene Names
; mAIM
UniProt Entry Name
CD5L_MOUSE
Uniprot Description
CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.
Protein type: Receptor, misc.; Secreted; Secreted, signal peptide
Research Articles on CD5L
Similar Products
Product Notes
The Mouse CD5L cd5l (Catalog #AAA1752777) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752777 ELISA Kit recognizes Mouse CD5L. It is sometimes possible for the material contained within the vial of "CD5L/CT 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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