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Immunofluorescence (IF) (MBS9601491 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Rabbit GLR Polyclonal Antibody | anti-GLR antibody

GLR Antibody

Gene Names
GCGR; GGR; GL-R
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity Purification
Synonyms
GLR; Polyclonal Antibody; GLR Antibody; Gcgr; GGR; GL R; GL-R; GLR_HUMAN; Glucagon receptor; Glucagon receptor precursor; MGC138246; anti-GLR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
GLR Antibody detects endogenous levels of total GLR.
Purity/Purification
Peptide affinity Purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GLR antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA(peptide) 1:20000-1:40000
Immunogen
A synthesized peptide derived from human GLR, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C.

Immunofluorescence (IF)

(MBS9601491 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunofluorescence (IF) (MBS9601491 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of GLR expression in HUVEC cell lysates)

Western Blot (WB) (Western blot analysis of GLR expression in HUVEC cell lysates)

Immunohistochemistry (IHC)

(MBS9601491 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (MBS9601491 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-GLR antibody
GLRB (Glycine receptor beta) is a neurotransmitter-gated ion channel concentrated within the spinal cord and brainstem. Expression is also observed in several upper brain regions including the cortex, cerebellum, hippocampus and amygdala. Binding of glycine to GLRB increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing), controlling spinal reflexes and locomotor behavior.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56 kDa
NCBI Official Full Name
glucagon receptor
NCBI Official Synonym Full Names
glucagon receptor
NCBI Official Symbol
GCGR
NCBI Official Synonym Symbols
GGR; GL-R
NCBI Protein Information
glucagon receptor
UniProt Protein Name
Glucagon receptor
UniProt Gene Name
GCGR
UniProt Synonym Gene Names
GL-R

NCBI Description

The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).[provided by RefSeq, Jan 2010]

Uniprot Description

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

Research Articles on GLR

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Product Notes

The GLR gcgr (Catalog #AAA9601491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GLR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500 IHC: 1:50-1:200 ELISA(peptide) 1:20000-1:40000. Researchers should empirically determine the suitability of the GLR gcgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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