Human G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) ELISA Kit | GNaI3 elisa kit

Human G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) ELISA Kit

Cat. Num. AAA455971

• Gene Names: GNAI3; 87U6; ARCND1
• Reactivity: Human
• Synonyms: G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3); Human G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3) ELISA Kit; GNaI3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GNaI3. No significant cross-reactivity or interference between GNaI3 and analogues was observed.
• Sequence Length: 354

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GNaI3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GNaI3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNaI3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GNaI3 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GNaI3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GNaI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GNaI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GNaI3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5729850
• NCBI GeneID: 2773
• NCBI Accession #: NP_006487.1
• NCBI GenBank Nucleotide #: NM_006496.3
• UniProt Accession #: P08754; P17539; Q5TZX1
• Molecular Weight: 40,532 Da
• NCBI Official Full Name: guanine nucleotide-binding protein G(k) subunit alpha
• NCBI Official Synonym Full Names: G protein subunit alpha i3
• NCBI Official Symbol: GNAI3
• NCBI Official Synonym Symbols: 87U6; ARCND1
• NCBI Protein Information: guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(k) subunit alpha
• UniProt Gene Name: GNAI3

NCBI Description

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]

Uniprot Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).