Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GLI1 antibody.)
Rabbit GLI1 Polyclonal Antibody | anti-GLI1 antibody
GLI1 Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GLI1 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using GLI1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
GLI1: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Interacts with KIF7. Interacts with ZIC1; the interaction enhances transcription activation. Amplified in glioblastoma cells. Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Oncoprotein; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 12q13.2-q13.3
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; metal ion binding; microtubule binding; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; smoothened signaling pathway; ventral midline development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; positive regulation of transcription, DNA-dependent; digestive tract morphogenesis; notochord regression; osteoblast differentiation; cerebellar cortex morphogenesis; dorsal/ventral pattern formation; pituitary gland development; positive regulation of cell proliferation; positive regulation of smoothened signaling pathway; epidermal cell differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; proximal/distal pattern formation; regulation of smoothened signaling pathway; lung development
Research Articles on GLI1
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Product Notes
The GLI1 gli1 (Catalog #AAA3010635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLI1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GLI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the GLI1 gli1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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