Western Blot (WB)
((LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.)
Rabbit GLD2 Polyclonal Antibody | anti-Gld2 antibody
GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Western Blot (WB)
((LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
((LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory. Ref.5
Catalytic activity: ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor: Magnesium or manganese
By similarity.
Subunit structure: Interacts with Fmr1 and eIF-4E. Ref.5
Subcellular location: Cytoplasm. Nucleus. Note: Found in neuronal cytoplasm, present in discrete neuronal particles associated with mRNA control, and in nuclear puncta. Ref.5
Tissue specificity: Expressed in the brain. Ref.5
Developmental stage: Expressed in larvae, pupae and adults. Ref.5
Sequence similarities: Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.Contains 1 PAP-associated domain.
Research Articles on Gld2
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Product Notes
The Gld2 gld2 (Catalog #AAA644631) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2) reacts with Hamster, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the Gld2 gld2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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