Mouse KCNQ1 Monoclonal Antibody | anti-KCNQ1 antibody

KCNQ1 Antibody: Alkaline Phosphatase

Cat. Num. AAA803537

• Gene Names: KCNQ1; LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Synonyms: KCNQ1; Monoclonal Antibody; KCNQ1 Antibody: Alkaline Phosphatase; ATFB1; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1; LQT; LQT1; RWS; SQT2; voltage gated potassium channel subunit KV7.1; anti-KCNQ1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S37A-10
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 676

• Applicable Applications for anti-KCNQ1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: Alkaline Phosphatase
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10. Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Western Blot (WB)

(Western Blot analysis of hamster T-CHO cell lysate showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-KCNQ1 antibody

Background Info: Detects ~75kDa.

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Kv7.1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. Kv7.1 is present in the cell membranes of cardiac muscle tissue and in inner ear neurons (3) among other tissues. In the cardiac cells, Kv7.1 mediates the IKs (or slow delayed rectifying K+) current that contributes to the repolarization of the cell, terminating the cardiac action potential and thereby the heart's contraction (4, 5).

Product Categories/Family for anti-KCNQ1 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Lang F., Vallon V., Knipper M., Wagenmann P. (2007) Am J Physiol. 293(4): C1187-1208. 4. Kurokawa J., et al. (2009) Channels (Austin). 3(1): 16-24. 5. Silva J., and Rudy Y. (2005) Circulation. 112(10): 1384- 1391.

NCBI and Uniprot Product Information

• NCBI GI #: 32479527
• NCBI GeneID: 3784
• NCBI Accession #: NP_000209.2
• NCBI GenBank Nucleotide #: NM_000218.2
• UniProt Accession #: P51787; O00347; O60607; O94787; Q14D14; Q7Z6G9; Q92960; Q9UMN8; Q9UMN9
• Molecular Weight: 61,474 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily KQT member 1 isoform 1
• NCBI Official Synonym Full Names: potassium voltage-gated channel, KQT-like subfamily, member 1
• NCBI Official Symbol: KCNQ1
• NCBI Official Synonym Symbols: LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1
• NCBI Protein Information: potassium voltage-gated channel subfamily KQT member 1; slow delayed rectifier channel subunit; voltage-gated potassium channel subunit Kv7.1; kidney and cardiac voltage dependend K+ channel; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
• UniProt Protein Name: Potassium voltage-gated channel subfamily KQT member 1
• Protein Family: KCNQ1 downstream neighbor protein
• UniProt Gene Name: KCNQ1
• UniProt Synonym Gene Names: KCNA8; KCNA9; KVLQT1
• UniProt Entry Name: KCNQ1_HUMAN

NCBI Description

This gene encodes a voltage-gated potassium channel required for repolarization phase of the cardiac action potential. This protein can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome 1 (also known as Romano-Ward syndrome), Jervell and Lange-Nielsen syndrome, and familial atrial fibrillation. This gene exhibits tissue-specific imprinting, with preferential expression from the maternal allele in some tissues, and biallelic expression in others. This gene is located in a region of chromosome 11 amongst other imprinted genes that are associated with Beckwith-Wiedemann syndrome (BWS), and itself has been shown to be disrupted by chromosomal rearrangements in patients with BWS. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Kv7.1: a voltage-gated potassium channel protein required for the repolarization phase of the cardiac action potential. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea. Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries. Three alternatively spliced isoforms have been described.

Protein type: Channel, potassium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: voltage-gated potassium channel complex; cytoplasmic vesicle membrane; basolateral plasma membrane; lysosome; early endosome; late endosome; plasma membrane

Molecular Function: calmodulin binding; protein binding; voltage-gated potassium channel activity; delayed rectifier potassium channel activity; protein phosphatase 1 binding

Biological Process: synaptic transmission; genetic imprinting; sensory perception of sound; positive regulation of heart rate; gene silencing; regulation of heart contraction; cardiac muscle contraction

Disease: Jervell And Lange-nielsen Syndrome 1; Beckwith-wiedemann Syndrome; Atrial Fibrillation, Familial, 3; Short Qt Syndrome 2; Long Qt Syndrome 1