Rabbit GLD2 Polyclonal Antibody | anti-GLD2 antibody

GLD2, NT (PAPD4, GLD2, Poly(A) RNA polymerase GLD2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2) (Biotin)

Cat. Num. AAA6302492

• Gene Names: PAPD4; GLD2; TUT2
• Reactivity: Human, Hamster, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GLD2; Polyclonal Antibody; NT (PAPD4; Poly(A) RNA polymerase GLD2; PAP-associated domain-containing protein 4; Terminal uridylyltransferase 2) (Biotin); PAPD4; Terminal uridylyltransferase 2; anti-GLD2 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GLD2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-95 amino acids from the N-terminal region of human GLD2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

((LEFT)Western blot analysis of GLD2 Antibody (N-term) in MCF-7 cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(MIDDLE)Western blot analysis of GLD2 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of GLD2 Antibody (N-term) in CHO cell line lysates (35ug/lane).GLD2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GLD2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GLD2 antibody

GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.

Product Categories/Family for anti-GLD2 antibody

Antibodies; Enzymes; Polymerase

References

Mullen, T.E., et al. Genes Dev. 22(1):50-65(2008). Kwak, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4407-4412(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 167555097
• NCBI GeneID: 167153
• NCBI Accession #: NP_001107865.1
• NCBI GenBank Nucleotide #: NM_001114393.2
• UniProt Accession #: Q6PIY7; Q86WZ2; Q8N927
• Molecular Weight: 55,502 Da
• NCBI Official Full Name: poly(A) RNA polymerase GLD2 isoform 1
• NCBI Official Synonym Full Names: poly(A) RNA polymerase D4, non-canonical
• NCBI Official Symbol: PAPD4
• NCBI Official Synonym Symbols: GLD2; TUT2
• NCBI Protein Information: poly(A) RNA polymerase GLD2
• UniProt Protein Name: Poly(A) RNA polymerase GLD2
• Protein Family: Glycerol 2-dehydrogenase
• UniProt Gene Name: PAPD4
• UniProt Synonym Gene Names: GLD2; hGLD-2; TUTase 2

Uniprot Description

PAPD4: Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation. Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.19; RNA processing; Transferase

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytosol

Molecular Function: ATP binding; metal ion binding; polynucleotide adenylyltransferase activity; protein binding

Biological Process: hemopoietic progenitor cell differentiation; hippocampus development; mRNA processing; neuron differentiation; retina development in camera-type eye; RNA polyadenylation