Rabbit anti-Human MBOAT7 Polyclonal Antibody | anti-MBOAT7 antibody

MBOAT7, ID (MBOAT7, BB1, LENG4, OACT7, Lysophospholipid acyltransferase 7, 1-acylglycerophosphatidylinositol O-acyltransferase, Bladder and breast carcinoma-overexpressed gene 1 protein, Leukocyte receptor cluster member 4, Lysophosphatidylinositol acyltr

Cat. Num. AAA6319430

• Gene Names: MBOAT7; BB1; LRC4; LENG4; LPIAT; MBOA7; OACT7; hMBOA-7
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBOAT7; Polyclonal Antibody; ID (MBOAT7; BB1; LENG4; OACT7; Lysophospholipid acyltransferase 7; 1-acylglycerophosphatidylinositol O-acyltransferase; Bladder and breast carcinoma-overexpressed gene 1 protein; Leukocyte receptor cluster member 4; Lysophosphatidylinositol acyltr; Lysophosphatidylinositol acyltransferase; Membrane-bound O-acyltransferase domain-containing protein 7; anti-MBOAT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MBOAT7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MBOAT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human MBOAT7.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MBOAT7 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MBOAT7 antibody detected the MBOAT7 protein (arrow).)

Related Product Information for anti-MBOAT7 antibody

This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-MBOAT7 antibody

Antibodies; Enzymes; Acyltransferase

References

Gijon, M.A., et al. J. Biol. Chem. 283(44):30235-30245(2008). Lee, H.C., et al. Mol. Biol. Cell 19(3):1174-1184(2008). Wende, H., et al. Immunogenetics 51 (8-9), 703-713 (2000): Fukunaga-Johnson, N., et al. Anticancer Res. 16 (3A), 1085-1090 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 225703081
• NCBI GeneID: 79143
• NCBI Accession #: NP_001139528.1; NP_001139554.1; NP_001139555.1; NP_077274.3
• NCBI GenBank Nucleotide #: NM_001146056.2
• UniProt Accession #: Q96N66; Q05DF0; Q7L5N2; Q99908; Q9BPV2; Q9BRE9; A9C4B6; B0V3I5; B4DQ87
• Molecular Weight: 52,765 Da
• NCBI Official Full Name: lysophospholipid acyltransferase 7 isoform 2
• NCBI Official Synonym Full Names: membrane bound O-acyltransferase domain containing 7
• NCBI Official Symbol: MBOAT7
• NCBI Official Synonym Symbols: BB1; LRC4; LENG4; LPIAT; MBOA7; OACT7; hMBOA-7
• NCBI Protein Information: lysophospholipid acyltransferase 7; LPLAT 7; h-mboa-7; lyso-PI acyltransferase; lysophosphatidylinositol acyltransferase; leukocyte receptor cluster (LRC) member 4; 1-acylglycerophosphatidylinositol O-acyltransferase; bladder and breast carcinoma-overexpr
• UniProt Protein Name: Lysophospholipid acyltransferase 7
• Protein Family: Lysophospholipid acyltransferase
• UniProt Gene Name: MBOAT7
• UniProt Synonym Gene Names: BB1; LENG4; OACT7; LPLAT 7; LPIAT; Lyso-PI acyltransferase; O-acyltransferase domain-containing protein 7; h-mboa-7
• UniProt Entry Name: MBOA7_HUMAN

Uniprot Description

Function: Acyltransferase which mediates the conversion of lysophosphatidylinositol (1-acylglycerophosphatidylinositol or LPI) into phosphatidylinositol (1,2-diacyl-sn-glycero-3-phosphoinositol or PI) (LPIAT activity). Prefers arachidonoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. Ref.1 Ref.7

Catalytic activity: Acyl-CoA + 1-acyl-sn-glycero-3-phosphatidylinositol = CoA + 1,2-diacyl-sn-glycero-3-phosphatidylinositol. Ref.1Acyl-[acyl-carrier-protein] + 1-acyl-sn-glycerol 3-phosphate = [acyl-carrier-protein] + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1

Enzyme regulation: Activity is inhibited by thimerosal.

Pathway: Lipid metabolism; phospholipid metabolism. Ref.1

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: Overexpressed in metastatic breast and bladder carcinomas relative to normal breast epithelium and urothelium. Ref.7

Sequence similarities: Belongs to the membrane-bound acyltransferase family.

Sequence caution: The sequence AAB37433.1 differs from that shown. Reason: Frameshift at positions 63, 93, 144 and 186.