Rabbit anti-Human GKAP1 Polyclonal Antibody | anti-GKAP1 antibody

GKAP1, CT (GKAP1, GKAP42, G kinase-anchoring protein 1, cGMP-dependent protein kinase-anchoring protein of 42kD) (PE)

Cat. Num. AAA6302379

• Gene Names: GKAP1; FKSG21; GKAP42
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GKAP1; Polyclonal Antibody; CT (GKAP1; GKAP42; G kinase-anchoring protein 1; cGMP-dependent protein kinase-anchoring protein of 42kD) (PE); cGMP-dependent protein kinase-anchoring protein of 42kD; anti-GKAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GKAP1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-367 amino acids from the C-terminal region of human GKAP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GKAP1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GKAP1 antibody detected the GKAP1 protein (arrow).)

Immunohistochemistry (IHC)

(GKAP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GKAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of GKAP1 (arrow) using rabbit polyclonal GKAP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GKAP1 gene.)

Related Product Information for anti-GKAP1 antibody

GKAP1 is a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-GKAP1 antibody

Antibodies; Protein Kinases

References

Lim, J., et al. Cell 125(4):801-814(2006). Sweetser, D.A., et al. Genes Chromosomes Cancer 44(3):279-291(2005). Yuasa, K., et al. J. Biol. Chem. 275(7):4897-4905(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 209915617
• NCBI GeneID: 80318
• NCBI Accession #: NP_001129425.1
• NCBI GenBank Nucleotide #: NM_001135953.1
• UniProt Accession #: Q5VSY0; Q96LI0; Q9BYI1; Q9BYI2; Q9H225
• Molecular Weight: 42,078 Da
• NCBI Official Full Name: G kinase-anchoring protein 1 isoform b
• NCBI Official Synonym Full Names: G kinase anchoring protein 1
• NCBI Official Symbol: GKAP1
• NCBI Official Synonym Symbols: FKSG21; GKAP42
• NCBI Protein Information: G kinase-anchoring protein 1; protein kinase anchoring protein GKAP42; cGMP-dependent protein kinase anchoring protein 42kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa
• UniProt Protein Name: G kinase-anchoring protein 1
• Protein Family: G kinase-anchoring protein
• UniProt Gene Name: GKAP1
• UniProt Synonym Gene Names: GKAP42; FKSG21
• UniProt Entry Name: GKAP1_HUMAN