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Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Rabbit anti-Human GJB5 Polyclonal Antibody | anti-GJB5 antibody

GJB5 Antibody (Center)

Gene Names
GJB5; CX31.1
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GJB5; Polyclonal Antibody; GJB5 Antibody (Center); Gap junction beta-5 protein; Connexin-311; Cx311; anti-GJB5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GJB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human GJB5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
89-119
Sequence Length
273
Applicable Applications for anti-GJB5 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC~~1:50~100
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(GJB5 Antibody (R104.Connexin) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).)

Western Blot (WB) (GJB5 Antibody (R104.Connexin) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).)
Related Product Information for anti-GJB5 antibody
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) such as GJB5 are thought to play crucial functional roles associated with these channels.
Product Categories/Family for anti-GJB5 antibody
References
Richard, G., et al., Nat. Genet. 20(4):366-369 (1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31088
NCBI Official Full Name
gap junction beta-5 protein
NCBI Official Synonym Full Names
gap junction protein, beta 5, 31.1kDa
NCBI Official Symbol
GJB5
NCBI Official Synonym Symbols
CX31.1
NCBI Protein Information
gap junction beta-5 protein
UniProt Protein Name
Gap junction beta-5 protein
UniProt Gene Name
GJB5
UniProt Synonym Gene Names
Cx31.1
UniProt Entry Name
CXB5_HUMAN

NCBI Description

This gene encodes a member of the beta-type (group I) connexin family. The encoded protein is a gap junction protein involved in intercellular communication related to epidermal differentiation and environmental sensing. This gene has been linked to non-small cell lung cancer. [provided by RefSeq, Nov 2012]

Uniprot Description

GJB5: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: connexon complex; integral to membrane

Biological Process: epidermis development; cell communication

Research Articles on GJB5

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Product Notes

The GJB5 gjb5 (Catalog #AAA9210641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 89-119. The GJB5 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GJB5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:50~100 WB~~1:1000. Researchers should empirically determine the suitability of the GJB5 gjb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GJB5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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