GITRL Polyclonal Antibody | anti-GITRL antibody

Rabbit Anti-Human GITRL (Glucocorticoid-induced TNF Receptor Ligand)

Cat. Num. AAA6120669

• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: GITRL; Polyclonal Antibody; Rabbit Anti-Human GITRL (Glucocorticoid-induced TNF Receptor Ligand); AITRL; Activation-inducible TNF-related Ligand; TNFSF18; anti-GITRL antibody

• Host: Host: Rabbit; Source: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GITRL. Detects a band of ~15-17kD by Western blot. Does not cross-react with mouse GITRL.
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-GITRL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA (direct and indirect): 1:5000-1:10,000; Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature; Immunohistochemistry: 5ug/ml
• Immunogen: Recombinant protein corresponding to human GITRL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS6120669 at 1:5000 dilution. 1: Human GITRL (His-tagged). 2: Human GITRL (DYKDDDDK-tagged). 3: Mouse GITRL (DYKDDDDK-tagged).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of GITRL using MBS6120669 in human uterus tissue (5ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Related Product Information for anti-GITRL antibody

GITRL is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g., testis, ..). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.