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Western Blot (WB) ((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat Apolipoprotein L4 Polyclonal Antibody | anti-APOL4 antibody

Goat anti-Apolipoprotein L4 Antibody

Gene Names
APOL4; APOLIV; APOL-IV
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Apolipoprotein L4; Polyclonal Antibody; Goat anti-Apolipoprotein L4 Antibody; Goat Anti-APOL4 Antibody; apolipoprotein L4; APOL4; apolipoprotein L; 4; APOL-IV; APOLIV; OTTHUMP00000028937; apolipoprotein L-IV; apolipoprotein L4 antibody; APOL4 antibody; 4 antibody; APOL-IV antibody; APOLIV antibody; OTTHUMP00000028937 antibody; apolipoprotein L-IV antibody; anti-APOL4 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ENLNELTHIHQSLK
Sequence Length
348
Applicable Applications for anti-APOL4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunogen
Peptide with sequence C-ENLNELTHIHQSLK, from the C Terminus of the protein sequence according to NP_085146.2; NP_663693.1.
Epitope
C Terminus
Note
This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,619 Da
NCBI Official Full Name
apolipoprotein L4 isoform 1
NCBI Official Synonym Full Names
apolipoprotein L4
NCBI Official Symbol
APOL4
NCBI Official Synonym Symbols
APOLIV; APOL-IV
NCBI Protein Information
apolipoprotein L4
UniProt Protein Name
Apolipoprotein L4
Protein Family
UniProt Gene Name
APOL4
UniProt Synonym Gene Names
ApoL-IV
UniProt Entry Name
APOL4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein. [provided by RefSeq, Jul 2008]

Uniprot Description

APOL4: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Belongs to the apolipoprotein L family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q11.2-q13.2

Cellular Component: extracellular space; intracellular membrane-bound organelle

Molecular Function: lipid binding

Biological Process: lipid metabolic process; lipid transport; lipoprotein metabolic process

Disease: Schizophrenia

Research Articles on APOL4

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Product Notes

The APOL4 apol4 (Catalog #AAA421696) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Apolipoprotein L4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein L4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Researchers should empirically determine the suitability of the APOL4 apol4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ENLNELTHIH QSLK. It is sometimes possible for the material contained within the vial of "Apolipoprotein L4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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