Rabbit GET4 Polyclonal Antibody | anti-GET4 antibody

GET4 Antibody

Cat. Num. AAA9631327

• Gene Names: GET4; CEE; TRC35; CGI-20; C7orf20
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GET4; Polyclonal Antibody; GET4 Antibody; C7orf20; CGI 20; CGI20; Conserved edge expressed protein; Conserved edge protein; Conserved edge-expressed protein; GET 4; GET4_HUMAN; Golgi to ER traffic protein 4 homolog (S. cerevisiae); Golgi to ER traffic protein 4 homolog; Transmembrane domain recognition complex 35 kDa subunit; Transmembrane domain recognition complex 35kDa; TRC35; UPF0363 protein C7orf20; anti-GET4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GET4 Antibody detects endogenous levels of GET4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GET4 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human GET4.
• Conjugation: Unconjugated
• Similarity: Belongs to the GET4 family.
• Post Translational Modifications: Ubiquitinated by RNF12, leading to proteasomal degradation. When unassembled from BAG6; ubiquitinylation is modulated by BAG6 quality control role and effectuated by RNF126.
• Subunit Structure: Component of the BAG6/BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35. Interacts with BAG6; the interaction is direct and localizes BAG6 to the cytosol.
• Function: As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GET4 antibody

Cytoplasm; Cytosol; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 38570062
• NCBI GeneID: 51608
• NCBI Accession #: NP_057033.2
• NCBI GenBank Nucleotide #: NM_015949.2
• UniProt Accession #: Q7L5D6; Q9UFC9; Q9Y309; A4D2Q1; B3KNC7
• Molecular Weight: 30,523 Da
• NCBI Official Full Name: Golgi to ER traffic protein 4 homolog
• NCBI Official Synonym Full Names: golgi to ER traffic protein 4 homolog (S. cerevisiae)
• NCBI Official Symbol: GET4
• NCBI Official Synonym Symbols: CEE; TRC35; CGI-20; C7orf20
• NCBI Protein Information: Golgi to ER traffic protein 4 homolog; H_NH1244M04.5; conserved edge expressed protein; conserved edge protein; conserved edge-expressed protein; transmembrane domain recognition complex 35 kDa subunit; transmembrane domain recognition complex, 35kDa
• UniProt Protein Name: Golgi to ER traffic protein 4 homolog
• Protein Family: Golgi to ER traffic protein
• UniProt Gene Name: GET4
• UniProt Synonym Gene Names: C7orf20; CEE; TRC35; TRC35
• UniProt Entry Name: GET4_HUMAN

Uniprot Description

GET4: Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Belongs to the GET4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cytosol

Biological Process: transport