GET4 recombinant protein

GET4, 1-327aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205662

• Gene Names: GET4; CEE; TRC35; CGI-20; C7orf20
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GET4; 1-327aa; Human; His tag; E Coli (Denatured); Golgi to ER traffic protein 4 homolog; Golgi to ER traffic protein 4 homolog (S. cerevisiae); C7orf20; CEE; CGI-20; TRC35; GET4 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.4M urea
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAAAAM AEQESARNGG RNRGGVQRVE GKLRASVEKG DYYEAHQMYR TLFFRYMSQS KHTEARELMY SGALLFFSHG QQNSAADLSM LVLESLEKAE VEVADELLEN LAKVFSLMDP NSPERVTFVS RALKWSSGGS GKLGHPRLHQ LLALTLWKEQ NYCESRYHFL HSADGEGCAN MLVEYSTSRG FRSEVDMFVA QAVLQFLCLK NKSSASVVFT TYTQKHPSIE DGPPFVEPLL NFIWFLLLAV DGGKLTVFTV LCEQYQPSLR RDPMYNEYLD RIGQLFFGVP PKQTSSYGGL LGNLLTSLMG SSEQEDGEES PSDGSPIELD
• Sequence Length: 327

• Applicable Applications for GET4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GET4 recombinant protein

GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting. Recombinant human GET4 protein, fused to His-tag at N-terminus, was expressed in E Coli.

References

Mariappan M., Li X., et al. (2010) Nature 466:1120-1124.

NCBI and Uniprot Product Information

• NCBI GI #: 38570062
• NCBI GeneID: 51608
• NCBI Accession #: NP_057033
• NCBI GenBank Nucleotide #: NM_015949.2
• UniProt Accession #: Q7L5D6; Q9UFC9; Q9Y309; A4D2Q1; B3KNC7
• Molecular Weight: 38.9 kDa (350aa)
• NCBI Official Full Name: Golgi to ER traffic protein 4 homolog
• NCBI Official Synonym Full Names: golgi to ER traffic protein 4
• NCBI Official Symbol: GET4
• NCBI Official Synonym Symbols: CEE; TRC35; CGI-20; C7orf20
• NCBI Protein Information: Golgi to ER traffic protein 4 homolog
• UniProt Protein Name: Golgi to ER traffic protein 4 homolog
• Protein Family: Golgi to ER traffic protein
• UniProt Gene Name: GET4
• UniProt Synonym Gene Names: C7orf20; CEE; TRC35; TRC35

Uniprot Description

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).