Rabbit anti-Bovine Gelatin, Skin Polyclonal Antibody | anti-Mmp9 antibody

Gelatin, Skin

Cat. Num. AAA625052

• Reactivity: Bovine
• Applications: ELISA
• Purity: Serum
• Synonyms: Gelatin; Skin; Polyclonal Antibody; Anti -Gelatin; anti-Mmp9 antibody

• Host: Rabbit
• Reactivity: Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes bovine skin gelatin.
• Purity/Purification: Serum
• Form/Format: Supplied as a liquid, 0.09% sodium azide

• Applicable Applications for anti-Mmp9 antibody: Suitable for use in ELISA. Other applications not tested.
• Application Notes: Dilution: ELISA: 1:1000-1:10,000; Coat gelatin antigen at 5-10ug/ml.; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Gelatin, bovine skin Type B
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Mmp9 antibody

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue. The charge on a gelatin molecule and its isoelectric point are primarily due to the carboxyl amino and guanidino groups on the side chains. Type A gelatin has 78-80 millimoles of free carboxyl groups per 100g of protein and a pI of 7.0-9.0; type B has 100-115 millimoles of free carboxyl groups per 100g of protein and a pI of 4.7-5.2. The pH of a 1.5% solution at 25°C is 3.8-5.5 for Type A and 5.0-7.5 for Type B. Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western Blot, ELISA, and Immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media for species differentiation. Additionally, as a biocompatible polymer, gelatin has been used as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for tissue engineering applications. Gelatin proteins are also used as additives in purified, recombinant proteins and antibodies. Animals or humans exposed to gelatins may invoke antibody response. Often the gelatin must be removed prior to such applications. Antibodies to gelatins were produced to detect the presence of gelatins in biolgocial samples and or to verify their removal during bioprocess development.

Product Categories/Family for anti-Mmp9 antibody

Antibodies; Abs to Collagen

NCBI and Uniprot Product Information

• NCBI GI #: 1173506
• NCBI GeneID: 81687
• UniProt Accession #: P50282
• NCBI Official Full Name: gelatinase B
• NCBI Official Synonym Full Names: matrix metallopeptidase 9
• NCBI Official Symbol: Mmp9
• NCBI Protein Information: matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp9
• UniProt Synonym Gene Names: MMP-9; GELB
• UniProt Entry Name: MMP9_RAT

NCBI Description

metalloproteinase involved in extracellular matrix remodeling and bone resorption [RGD, Feb 2006]

Uniprot Description

Function: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments

By similarity.

Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V.

Cofactor: Binds 2 zinc ions per subunit

By similarity.Binds 3 calcium ions per subunit

By similarity.

Subunit structure: Exists as monomer or homodimer; disulfide-linked

By similarity. Exists also as heterodimer with a 25 kDa protein

By similarity. Interacts with ECM1

By similarity.

Subcellular location: Secreted › extracellular space › extracellular matrix

Probable.

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification: N- and O-glycosylated

By similarity.

Sequence similarities: Belongs to the peptidase M10A family.Contains 3 fibronectin type-II domains.Contains 4 hemopexin repeats.