Mouse Matrix metalloproteinase-9 ELISA Kit | Mmp9 elisa kit

Mouse Matrix metalloproteinase-9 ELISA Kit

Cat. Num. AAA2886011

• Gene Names: Mmp9; Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9
• Reactivity: Mouse
• Synonyms: Matrix metalloproteinase-9; Mouse Matrix metalloproteinase-9 ELISA Kit; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; Mmp9; Clg4b; 3.4.24.35; Mmp9 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Matrix metalloproteinase-9,MMP-9
• Sequence Length: 730

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.086 ng/mL
• Intra-assay Precision: <=7.5%
• Inter-assay Precision: <=9.5%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Mmp9 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7305277
• NCBI GeneID: 17395
• NCBI Accession #: NP_038627.1
• NCBI GenBank Nucleotide #: NM_013599.4
• UniProt Accession #: P41245; Q06788; Q80XI8; Q9DC02
• Molecular Weight: 80,535 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9
• NCBI Official Symbol: Mmp9
• NCBI Official Synonym Symbols: Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9
• NCBI Protein Information: matrix metalloproteinase-9
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp9
• UniProt Synonym Gene Names: Clg4b; MMP-9; GELB
• UniProt Entry Name: MMP9_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Protease; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.35

Cellular Component: extracellular matrix; extracellular region; extracellular space; protein complex; proteinaceous extracellular matrix

Molecular Function: endopeptidase activity; fibronectin binding; hydrolase activity; identical protein binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protein complex binding; zinc ion binding

Biological Process: collagen catabolic process; embryo implantation; extracellular matrix organization and biogenesis; heart development; leukocyte migration; negative regulation of apoptosis; negative regulation of fibroblast proliferation; ossification; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of DNA binding; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of leukocyte migration; positive regulation of protein amino acid phosphorylation; positive regulation of synaptic plasticity; protein oligomerization; proteolysis; response to drug; response to oxidative stress; skeletal development; tissue remodeling; transformation of host cell by virus