Western Blot (WB)
(WB Suggested Anti-GEFT Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
Rabbit GEFT Polyclonal Antibody | anti-ARHGEF25 antibody
GEFT antibody - C-terminal region
Gene Names
ARHGEF25; GEFT; p63RhoGEF
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
GEFT; Polyclonal Antibody; GEFT antibody - C-terminal region; anti-ARHGEF25 antibody
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: RLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQAPHD
Sequence Length
580
Applicable Applications for anti-ARHGEF25 antibody
Western Blot (WB)
Homology
Cow: 92%; Dog: 85%; Guinea Pig: 85%; Horse: 85%; Human: 100%; Mouse: 85%; Pig: 100%; Rabbit: 85%; Rat: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human GEFT
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(WB Suggested Anti-GEFT Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
Western Blot (WB)
(WB Suggested Anti-GEFT Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)
Related Product Information for anti-ARHGEF25 antibody
This is a rabbit polyclonal antibody against GEFT. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange.The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 [PubMed 11861769]).[supplied by OMIM].
Target Description: The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange.The Rho family of small GTPases act as molecular switches to control a wide range of cellular processes. Guanine nucleotide exchange factors (GEFs), like GEFT, activate Rho GTPases by accelerating GDP/GTP exchange (Souchet et al., 2002 [PubMed 11861769]).[supplied by OMIM].
Product Categories/Family for anti-ARHGEF25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64kDa
NCBI Official Full Name
rho guanine nucleotide exchange factor 25 isoform 1
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 25
NCBI Official Symbol
ARHGEF25
NCBI Official Synonym Symbols
GEFT; p63RhoGEF
NCBI Protein Information
rho guanine nucleotide exchange factor 25
UniProt Protein Name
Rho guanine nucleotide exchange factor 25
Protein Family
UniProt Gene Name
ARHGEF25
UniProt Synonym Gene Names
GEFT
NCBI Description
Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis ().
Research Articles on ARHGEF25
Similar Products
Product Notes
The ARHGEF25 arhgef25 (Catalog #AAA3211309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GEFT antibody - C-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GEFT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ARHGEF25 arhgef25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RLGDQAQGST HTPINGSLPS LLLSPKGEVA RALLPLDKQA LGDIPQAPHD. It is sometimes possible for the material contained within the vial of "GEFT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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