Rabbit anti-Human, Rat GCK/GLK Polyclonal Antibody | anti-GCK/GLK antibody

GCK/GLK Polyclonal Antibody

Cat. Num. AAA9419895

• Gene Names: MAP4K2; GCK; BL44; RAB8IP
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: GCK/GLK; Polyclonal Antibody; GCK/GLK Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase kinase 2 (EC 2.7.11.1) (B lymphocyte serine/threonine-protein kinase) (Germinal center kinase) (GC kinase) (MAPK/ERK kinase kinase kinase 2) (MEK kinase kinase 2) (MEKKK 2) (Rab8-interacting protein) Mitogen-activated protein kinase kinase kinase kinase; anti-GCK/GLK antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: GCK/GLK Polyclonal Antibody detects endogenous levels of GCK/GLK
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-GCK/GLK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Synthesized peptide derived from human GCK/GLK. at AA range: 221-270
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western Blot analysis of 1, 293T 2, hela cells using primary antibody diluted at 1:1000 ( degree C overnight). Secondary antibody£ºGoat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25¡£C, 1 hour))

Product Categories/Family for anti-GCK/GLK antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 808175990
• NCBI GeneID: 5871
• NCBI Accession #: NP_001294919.1
• NCBI GenBank Nucleotide #: NM_001307990.1
• UniProt Accession #: Q12851; Q86VU3
• Molecular Weight: 100 kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 2 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 2
• NCBI Official Symbol: MAP4K2
• NCBI Official Synonym Symbols: GCK; BL44; RAB8IP
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 2
• UniProt Gene Name: MAP4K2
• UniProt Synonym Gene Names: GCK; RAB8IP; GC kinase; MEK kinase kinase 2; MEKKK 2

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GCK: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A. Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. The tumor necrosis factor (TNF), as well as endotoxins and proinflammatory stimuli such as polyinosine- polycytidine (poly(IC)), lipopolysaccharides (LPS), peptidoglycan (PGN), flagellin, or lipid A activate MAP4K2 by promoting its autophosphorylation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: EC 2.7.11.1; KHS subfamily; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm

Molecular Function: ATP binding; MAP kinase kinase kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein serine/threonine kinase activity

Biological Process: activation of JNK activity; immune response; JNK cascade; positive regulation of JNK cascade; protein amino acid phosphorylation; regulation of apoptosis; regulation of mitotic cell cycle