Rabbit anti-Human GBP1 Polyclonal Antibody | anti-GBP1 antibody

GBP1 Polyclonal Antibody

Cat. Num. AAA3011835

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GBP1; Polyclonal Antibody; GBP1 Polyclonal Antibody; Interferon-induced guanylate-binding protein 1; GTP-binding protein 1; GBP-1; HuGBP-1; Guanine nucleotide-binding protein 1; anti-GBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GBP1 polyclonal antibody detects endogenous levels of GBP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 592

• Applicable Applications for anti-GBP1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human GBP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extract of 5637 cell lines, using GBP1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using GBP1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-GBP1 antibody

GBP1 (guanylate binding protein 1) is a 592 amino acid protein member of the GTPase protein family and is able to bind specifically to guanine nucleotides such as GMP, GDP and GTP. GMP is hydrolyzed to GTP in two consecutive cleavage step, both of which are carried out by GBP1. Localized to the cytoplasm, GBP1 is expressed in endothelial cells of the vascular system and is induced by IFN-gamma during macrophage induction. GBP1 is thought to regulate the expression of MMP-1, which mediates the proliferation and invasiveness of endothelial cells. GBP1 plays a key role in regulating inflammatory cytokines and provides protection against vesicular stomatitis and encephalomyocarditis viruses. GBP1 expression is highly induced in the vessels of skin diseases such as psoriasis and Kaposis sarcoma, making it a novel cellular activation marker that characterizes inflammatory cytokines of endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 166706903
• NCBI GeneID: 2633
• NCBI Accession #: NP_002044.2
• NCBI GenBank Nucleotide #: NP_002044.2
• UniProt Accession #: P32455
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: guanylate-binding protein 1
• NCBI Official Synonym Full Names: guanylate binding protein 1
• NCBI Official Symbol: GBP1
• NCBI Protein Information: guanylate-binding protein 1
• UniProt Protein Name: Interferon-induced guanylate-binding protein 1
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP1
• UniProt Synonym Gene Names: GBP-1; HuGBP-1
• UniProt Entry Name: GBP1_HUMAN

NCBI Description

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008]

Uniprot Description

GBP1: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Vesicle; Cell adhesion; Hydrolase

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: Golgi membrane; extracellular region; cytosol

Molecular Function: GTPase activity; identical protein binding; GTP binding

Biological Process: metabolic process; cytokine and chemokine mediated signaling pathway; defense response to virus