Rabbit anti-Human GBAS Polyclonal Antibody | anti-GBAS antibody

GBAS, ID (GBAS, NIPSNAP2, Protein NipSnap homolog 2, Glioblastoma-amplified sequence) (FITC)

Cat. Num. AAA6301426

• Gene Names: GBAS; NIPSNAP2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GBAS; Polyclonal Antibody; ID (GBAS; NIPSNAP2; Protein NipSnap homolog 2; Glioblastoma-amplified sequence) (FITC); Glioblastoma-amplified sequence; anti-GBAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GBAS antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GBAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-170 amino acids from the Central region of human GBAS.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GBAS Antibody (Center) in Ramos cell line lysates (35ug/lane).GBAS (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GBAS Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GBAS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GBAS Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GBAS antibody

Chromosomal region 7p12, which contains GBAS, is amplified in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumor. The predicted 286-amino acid protein contains a signal peptide, a transmembrane domain, and 2 tyrosine phosphorylation sites. The GBAS transcript is expressed most abundantly in heart and skeletal muscle. GBAS protein might be involved in vesicular transport.

Product Categories/Family for anti-GBAS antibody

Antibodies; Proteins

References

Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000). Seroussi, E., et al. Gene 212(1):13-20(1998). Wang, X.Y., et al. Genomics 49(3):448-451(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 17380133
• NCBI GeneID: 2631
• UniProt Accession #: O75323; O43801; Q53X96; C9IYJ3
• Molecular Weight: 29,053 Da
• NCBI Official Full Name: Protein NipSnap homolog 2
• NCBI Official Synonym Full Names: glioblastoma amplified sequence
• NCBI Official Symbol: GBAS
• NCBI Official Synonym Symbols: NIPSNAP2
• NCBI Protein Information: protein NipSnap homolog 2; 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 2
• UniProt Protein Name: Protein NipSnap homolog 2
• Protein Family: Protein
• UniProt Gene Name: GBAS
• UniProt Synonym Gene Names: NIPSNAP2; NipSnap2
• UniProt Entry Name: NIPS2_HUMAN

NCBI Description

This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. The encoded protein is localized to mitochondria and plays a role in oxidative phosphorylation. A pseudogene of this gene is located on the long arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

GBAS: a member of the NipSnap family of proteins that may be involved in vesicular transport. The encoded protein is localized to mitochondria and plays a role in oxidative phosphorylation. A pseudogene of this gene is located on the long arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]

Protein type: Autophagy; Cell surface

Chromosomal Location of Human Ortholog: 7p12

Cellular Component: membrane; mitochondrion; integral to plasma membrane

Molecular Function: protein binding

Biological Process: ATP biosynthetic process; oxidative phosphorylation