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Western Blot (WB) (Anti-BCAT2 antibody, MBS175502, Western blottingAll lanes: Anti BCAT2 (MBS175502) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: CEM Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD )

Rabbit anti-Human BCAT2 Polyclonal Antibody | anti-BCAT2 antibody

Anti-BCAT2 antibody

Gene Names
BCAT2; BCAM; BCT2; PP18; BCATM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
BCAT2; Polyclonal Antibody; Anti-BCAT2 antibody; Branched-chain-amino-acid aminotransferase; mitochondrial(BCAT(m)); branched chain amino-acid transaminase 2; mitochondrial; BCAM antibody; BCAT 2 antibody; BCAT(m) antibody; BCAT2 antibody; BCAT2_HUMAN antibody; BCATM antibody; BCT2 antibody; Branched chain aminotransferase 2; mitochondrial antibody; Branched-chain-amino-acid aminotransferase antibody; ECA40 antibody; Placental protein 18 antibody; PP18 antibody; anti-BCAT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
392
Applicable Applications for anti-BCAT2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT2 (377-392aa KEIQYGIRAHEWMFPV).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-BCAT2 antibody, MBS175502, Western blottingAll lanes: Anti BCAT2 (MBS175502) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: CEM Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD )

Western Blot (WB) (Anti-BCAT2 antibody, MBS175502, Western blottingAll lanes: Anti BCAT2 (MBS175502) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: CEM Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD )

Immunohistochemistry (IHC)

(Anti-BCAT2 antibody, MBS175502, IHC(P)IHC(P): Human Colon Cancer Tissue )

Immunohistochemistry (IHC) (Anti-BCAT2 antibody, MBS175502, IHC(P)IHC(P): Human Colon Cancer Tissue )
Related Product Information for anti-BCAT2 antibody
Description: Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, mitochondrial(BCAT2) detection. Tested with WB, IHC-P in Human.
Background: BCAT2, branched-chain aminotransferase 2, encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. The human BCAT2 gene is mapped to 19q13.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
587
UniProt Accession #
Molecular Weight
33,777 Da
NCBI Official Full Name
Branched-chain-amino-acid aminotransferase, mitochondrial
NCBI Official Synonym Full Names
branched chain amino-acid transaminase 2, mitochondrial
NCBI Official Symbol
BCAT2
NCBI Official Synonym Symbols
BCAM; BCT2; PP18; BCATM
NCBI Protein Information
branched-chain-amino-acid aminotransferase, mitochondrial; placental protein 18; branched chain aminotransferase 2, mitochondrial
UniProt Protein Name
Branched-chain-amino-acid aminotransferase, mitochondrial
UniProt Gene Name
BCAT2
UniProt Synonym Gene Names
BCATM; BCT2; ECA40; BCAT(m); PP18
UniProt Entry Name
BCAT2_HUMAN

NCBI Description

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

BCAT2: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. Homodimer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Mitochondrial; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 2.6.1.42; Transferase

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: branched-chain-amino-acid transaminase activity

Biological Process: valine metabolic process; branched chain family amino acid catabolic process; leucine metabolic process; isoleucine catabolic process; branched chain family amino acid biosynthetic process

Research Articles on BCAT2

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Product Notes

The BCAT2 bcat2 (Catalog #AAA175502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCAT2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCAT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the BCAT2 bcat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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