Rabbit GATA4 Polyclonal Antibody | anti-GATA4 antibody

GATA4 (R306) Polyclonal Antibody

Cat. Num. AAA3002017

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GATA4; Polyclonal Antibody; GATA4 (R306) Polyclonal Antibody; Transcription factor GATA-4; GATA-binding factor 4; GATA 4; anti-GATA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GATA4 (R306) polyclonal antibody detects endogenous levels of GATA4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 442

• Applicable Applications for anti-GATA4 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 280-320 of Human GATA4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GATA4 (R306) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Related Product Information for anti-GATA4 antibody

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

NCBI and Uniprot Product Information

• NCBI GI #: 33188461
• NCBI GeneID: 2626
• NCBI Accession #: NP_002043.2
• NCBI GenBank Nucleotide #: NP_002043.2
• UniProt Accession #: P43694
• Molecular Weight: ~ 46kDa
• NCBI Official Full Name: transcription factor GATA-4 isoform 2
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; transcription activator binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; negative regulation of autophagy; positive regulation of transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; regulation of transcription, DNA-dependent; cell-cell signaling; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to estrogen stimulus; response to mechanical stimulus; endoderm formation; positive regulation of cardioblast differentiation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; blood coagulation; endoderm development

Disease: Ventricular Septal Defect 1; Tetralogy Of Fallot; Atrial Septal Defect 2; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease