Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Rat GATA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat GATA Binding Protein 1 Polyclonal Antibody | anti-GATA1 antibody
Polyclonal Antibody to GATA Binding Protein 1 (GATA1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DTASSSTSP NAATAAATAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGS SPYASWAYSK TALYPASTVC PSHEDAPSQT LEDPDGKNNN TFLETLKTER LSPDLLTLGT ALPTSLPVTS SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Rat GATA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human HL60 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Rat GATA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI Description
transcription factor that may regulate transcription of serine dehydratase [RGD, Feb 2006]
Uniprot Description
GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: Xq12
Cellular Component: nucleus; transcription factor complex; transcriptional repressor complex
Molecular Function: chromatin binding; chromatin DNA binding; DNA binding; DNA binding transcription factor activity; DNA binding, bending; p53 binding; sequence-specific DNA binding; zinc ion binding
Biological Process: basophil differentiation; cell development; cell-cell signaling; elevation of cytosolic calcium ion concentration; embryonic hemopoiesis; eosinophil differentiation; erythrocyte development; erythrocyte differentiation; homeostasis of number of cells within a tissue; in utero embryonic development; male gonad development; megakaryocyte differentiation; myeloid cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; platelet formation; positive regulation of erythrocyte differentiation; positive regulation of mast cell degranulation; positive regulation of osteoblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; Sertoli cell development; transcription from RNA polymerase II promoter
Research Articles on GATA1
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Product Notes
The GATA1 gata1 (Catalog #AAA2027778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to GATA Binding Protein 1 (GATA1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GATA Binding Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DTASSSTSP NAATAAATAL AYYREAEAYR HSPVFQVYPL LNSMEGIPGS SPYASWAYSK TALYPASTVC PSHEDAPSQT LEDPDGKNNN TFLETLKTER LSPDLLTLGT ALPTSLPVTS SAYGGADFPS PFFSPTGSPL SSAAYSSPKF HGSLPLAPCE ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRMIV SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ. It is sometimes possible for the material contained within the vial of "GATA Binding Protein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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