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Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10% SDS PAGE gel and GAPDH antibody at a dilution of 1:10000)
Rabbit GAPDH Polyclonal Antibody | anti-GAPDH antibody
GAPDH Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
GAPDH; Polyclonal Antibody; GAPDH Antibody; Affinity Purified Rabbit Polyclonal GAPDH Antibody; Polyclonal GAPDH antibody; Anti-GAPDH antibody; GAPD antibody; G3PD antibody; GAPDH antibody; MGC88685 antibody; glyceraldehyde-3-phosphate dehydrogenase antibody; glyceraldehyde 3-phosphate dehydrogenase antibody; aging-associated gene 9 protein antibody; peptidyl-cysteine S-nitrosylase GAPDH antibody; anti-GAPDH antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GAPDH antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:1000
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:5000-1:20000
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:5000-1:20000
Immunogen
GAPDH antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 335 of GAPDH
Cross-Reactivity
Drosophila, Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10% SDS PAGE gel and GAPDH antibody at a dilution of 1:10000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10% SDS PAGE gel and GAPDH antibody at a dilution of 1:10000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: drosophila) using a 10% SDS PAGE gel and GAPDH antibody at a dilution of 1:5000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: drosophila) using a 10% SDS PAGE gel and GAPDH antibody at a dilution of 1:5000)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded salivary gland cancer tissue using GAPDH antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded salivary gland cancer tissue using GAPDH antibody at a dilution of 1:500)
Related Product Information for anti-GAPDH antibody
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Product Categories/Family for anti-GAPDH antibody
Similar Products
Product Notes
The GAPDH (Catalog #AAA5311745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:1000 IF: 1:100-1:500 ICC: 1:100-1:500 WB: 1:5000-1:20000. Researchers should empirically determine the suitability of the GAPDH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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