Typical Testing Data/Standard Curve (for reference only)
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General Bovine Serum Albumin (BSA) ELISA Kit | BSA elisa kit
Bovine Serum Albumin (BSA) ELISA Kit
Reactivity
General
Synonyms
Bovine Serum Albumin (BSA); Bovine Serum Albumin (BSA) ELISA Kit; Bovine Albumin; Fraction V; BSA elisa kit
Reactivity
General
Specificity
This assay has high sensitivity and excellent specificity for detection of BSA. No significant cross-reactivity or interference between BSA and analogues was observed.
Sequence Length
607
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
370.4-30,000ng/mL
Sensitivity
< 161.2ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BSA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BSA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for BSA elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BSA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BSA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BSA and unlabeled BSA (Standards or samples) with the pre-coated antibody specific to BSA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BSA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BSA in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BSA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BSA and unlabeled BSA (Standards or samples) with the pre-coated antibody specific to BSA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BSA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BSA in the sample.
Product Categories/Family for BSA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,293 Da
NCBI Official Full Name
bovine serum albumin
NCBI Official Symbol
ALB
NCBI Protein Information
serum albumin
UniProt Protein Name
Serum albumin
UniProt Gene Name
ALB
UniProt Entry Name
ALBU_BOVIN
Uniprot Description
albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc
Protein type: Secreted; Carrier; Secreted, signal peptide
Cellular Component: protein complex; cytoplasm; extracellular region; nucleus
Molecular Function: toxin binding; DNA binding; metal ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding
Biological Process: retinal homeostasis; maintenance of mitochondrion localization; transport; hemolysis by symbiont of host red blood cells; cellular response to starvation; negative regulation of apoptosis
Research Articles on BSA
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Product Notes
The General BSA alb (Catalog #AAA2000242) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2000242 ELISA Kit recognizes General BSA. It is sometimes possible for the material contained within the vial of "Bovine Serum Albumin (BSA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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