Rabbit anti-Zebrafish GAPDH Polyclonal Antibody | anti-GAPDH antibody

GAPDH Rabbit pAb

Cat. Num. AAA8808240

• Gene Names: GAPDH; G3PD; GAPD
• Reactivity: Zebrafish
• Synonyms: GAPDH; Polyclonal Antibody; GAPDH Rabbit pAb; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphoate dehycdrogenase; MGC102544; anti-GAPDH antibody

• Host: Rabbit
• Reactivity: Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: PBS with 0.02% sodium azide and 50% glycerol pH7.4.
• Concentration: 1mg/ml (varies by lot)

• Species: Human
• Immunogen: Recombinant Protein
• Preparation and Storage: Store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Zebrafish skeletal muscle with GAPDH Rabbit pAb (Zebrafish Specific) diluted at 1:5,000.)

Related Product Information for anti-GAPDH antibody

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. 

NCBI and Uniprot Product Information

• NCBI GI #: 378404908
• NCBI GeneID: 2597
• NCBI Accession #: NP_001243728.1
• NCBI GenBank Nucleotide #: NM_001256799.1
• UniProt Accession #: P04406; P00354; Q53X65
• Molecular Weight: 36,053 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH
• UniProt Entry Name: G3P_HUMAN

NCBI Description

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.2.1.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: microtubule cytoskeleton; nuclear membrane; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; lipid particle; ribonucleoprotein complex; cytosol; nucleus; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: neuron apoptosis; glycolysis; protein stabilization; negative regulation of translation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; microtubule cytoskeleton organization and biogenesis; gluconeogenesis