Typical Testing Data/Standard Curve (for reference only)
Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) CLIA Kit | MPIF2 clia kit
Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) CLIA Kit
Gene Names
Ccl24; CKb-6; MPIF-2; Scya24
Reactivity
Mouse
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
Myeloid Progenitor Inhibitory Factor 2 (MPIF2); Mouse Myeloid Progenitor Inhibitory Factor 2 (MPIF2) CLIA Kit; Myeloid Progenitor Inhibitory Factor 2; CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24; MPIF2 clia kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2).
No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.
No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.
Sequence Length
119
Applicable Applications for MPIF2 clia kit
Chemiluminescence Immunoassay (CLIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
2.74-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.97pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloid Progenitor Inhibitory Factor 2 (MPIF2) level in the sample or standard.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C motif chemokine 24
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 24
NCBI Official Symbol
Ccl24
NCBI Official Synonym Symbols
CKb-6; MPIF-2; Scya24
NCBI Protein Information
C-C motif chemokine 24
UniProt Protein Name
C-C motif chemokine 24
UniProt Gene Name
Ccl24
UniProt Synonym Gene Names
Scya24
UniProt Entry Name
CCL24_MOUSE
Uniprot Description
CCL24: Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; cytokine activity
Biological Process: regulation of cell shape; positive regulation of angiogenesis; positive regulation of actin filament polymerization; eosinophil chemotaxis; immune response; cytoskeleton organization and biogenesis; positive regulation of endothelial cell proliferation; inflammatory response; chemotaxis; positive regulation of cell migration; positive regulation of inflammatory response
Research Articles on MPIF2
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Product Notes
The Mouse MPIF2 ccl24 (Catalog #AAA2708830) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708830 CLIA Kit recognizes Mouse MPIF2. AAA Biotech's Myeloid Progenitor Inhibitory Factor 2 (MPIF2) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the MPIF2 ccl24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myeloid Progenitor Inhibitory Factor 2 (MPIF2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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