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Western Blot (WB) (Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS6009540. GALNT2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human GALNT2 Polyclonal Antibody | anti-GALNT2 antibody

GALNT2, NT (GALNT2, Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, Protein-UDP acetylgalactosaminyltransferase 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide N-acetylgalactosaminyltransferase

Gene Names
GALNT2; GalNAc-T2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
GALNT2; Polyclonal Antibody; NT (GALNT2; Polypeptide N-acetylgalactosaminyltransferase 2; Polypeptide GalNAc transferase 2; Protein-UDP acetylgalactosaminyltransferase 2; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2; Polypeptide N-acetylgalactosaminyltransferase; Anti -GALNT2; anti-GALNT2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-GALNT2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
GALNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human GALNT2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS6009540. GALNT2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western Blot analysis in Hela cell line lysates (35ug/lane) using MBS6009540. GALNT2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6009540, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6009540 for immunohistochemistry)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6009540, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6009540 for immunohistochemistry)

Testing Data

Testing Data
Related Product Information for anti-GALNT2 antibody
GALNT2 encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases.
Product Categories/Family for anti-GALNT2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
64,733 Da
NCBI Official Full Name
GALNT2 protein
NCBI Official Synonym Full Names
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
NCBI Official Symbol
GALNT2
NCBI Official Synonym Symbols
GalNAc-T2
NCBI Protein Information
polypeptide N-acetylgalactosaminyltransferase 2; pp-GaNTase 2; UDP-GalNAc transferase 2; polypeptide GalNAc transferase 2; protein-UDP acetylgalactosaminyltransferase 2; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2
UniProt Protein Name
Polypeptide N-acetylgalactosaminyltransferase 2
UniProt Gene Name
GALNT2
UniProt Synonym Gene Names
GalNAc-T2
UniProt Entry Name
GALT2_HUMAN

NCBI Description

This gene encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases. [provided by RefSeq, Jul 2008]

Uniprot Description

GALNT2: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.41

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; perinuclear region of cytoplasm; integral to Golgi membrane

Molecular Function: polypeptide N-acetylgalactosaminyltransferase activity; manganese ion binding; carbohydrate binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; protein amino acid O-linked glycosylation via threonine; immunoglobulin biosynthetic process; post-translational protein modification; protein amino acid O-linked glycosylation via serine

Research Articles on GALNT2

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Product Notes

The GALNT2 galnt2 (Catalog #AAA6009540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GALNT2, NT (GALNT2, Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, Protein-UDP acetylgalactosaminyltransferase 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide N-acetylgalactosaminyltransferase reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GALNT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the GALNT2 galnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GALNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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