GALNT2 recombinant protein

GALNT2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121530

• Gene Names: GALNT2; GalNAc-T2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GALNT2; GALNT2 Protein; Human; Recombinant (His Tag); Human GALNT2/GalNAc-T2 Protein (His Tag); GalNAc-T2 Protein; polypeptide N-acetylgalactosaminyltransferase 2; GALNT2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 150mM NaCl, 0.05% Brij-35, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys52-Gln571

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GALNT2 (NP_004472.1)(Lys52-Gln571) was expressed with an N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GALNT2 recombinant protein

Background: GALNT2, also known as GalNAc-T2, is a member of the GalNAc-transferases family. Members of this family transfer an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. GALNT2 may play a role in lipid metabolism. It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT2 has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.

References

Bennett EP. et al., 1998, Glycobiology. 8 (6): 547-55. White T. et al., 1995, J Biol Chem. 270 (41): 24156-65. Bennett EP. et al., 1996, J Biol Chem. 271 (29): 17006-12. Holleboom AG. et al., 2011, Cell Metab. 14 (6): 811-8.

NCBI and Uniprot Product Information

• NCBI GI #: 4758412
• NCBI GeneID: 2590
• NCBI Accession #: NP_004472.1
• NCBI GenBank Nucleotide #: NM_004481.4
• UniProt Accession #: Q10471; Q9NPY4; A8K1Y3; B7Z8V8; C5HU00
• Molecular Weight: 30,256 Da
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 2
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 2
• NCBI Official Symbol: GALNT2
• NCBI Official Synonym Symbols: GalNAc-T2
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 2
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 2
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT2
• UniProt Synonym Gene Names: GalNAc-T2; pp-GaNTase 2
• UniProt Entry Name: GALT2_HUMAN

NCBI Description

This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]