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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Galectin 3 (GAL3) Polyclonal Antibody | anti-GAL3 antibody

Polyclonal Antibody to Galectin 3 (GAL3)

Gene Names
Lgals3; L-34; CBP30; gal-3; AGE-R3
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Galectin 3 (GAL3); Polyclonal Antibody; Polyclonal Antibody to Galectin 3 (GAL3); anti-GAL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAL3. It has been selected for its ability to recognize GAL3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.77mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-MADGFSLNDA LAGSGNPNPQ GWPGAWGNQP GAGGYPGASY PGAYPGQAPP GGYPGQAPPS AYPGPTGPSA YPGPTAPGAY PGPTAPGAFP GQPGGPGAYP SAPGAYPSAP GAYPATGPFG APTGPLTVPY DMPLPGGVMP RMLITIIGTV KPNANSITLN FKKGNDIAFH FNPRFNENNR RVIVCNTKQD NNWGREERQS AFPFESGKPF KIQVLVEADH FKVAVNDVHL LQYNHRMKNL REISQLGIIG DITLTSASHA MI
Sequence Length
250
Applicable Applications for anti-GAL3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAL3 (Met1~Ile262) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040794
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded brain tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded brain tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,202 Da
NCBI Official Full Name
galectin-3
NCBI Official Synonym Full Names
galectin 3
NCBI Official Symbol
Lgals3
NCBI Official Synonym Symbols
L-34; CBP30; gal-3; AGE-R3
NCBI Protein Information
galectin-3
UniProt Protein Name
Galectin-3
UniProt Gene Name
Lgals3
UniProt Synonym Gene Names
Gal-3; CBP 35

NCBI Description

lectin molecule that binds IgE; may have role in immune function [RGD, Feb 2006]

Uniprot Description

galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Cell surface; Extracellular matrix; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15p14

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; extracellular exosome; extracellular matrix; extracellular region; extracellular space; immunological synapse; membrane; mitochondrial inner membrane; nucleus; plasma membrane; proteinaceous extracellular matrix; spliceosomal complex

Molecular Function: advanced glycation end-product receptor activity; carbohydrate binding; chemoattractant activity; disaccharide binding; IgE binding; laminin binding; monosaccharide binding; RNA binding

Biological Process: cell differentiation; eosinophil chemotaxis; epithelial cell differentiation; extracellular matrix organization; innate immune response; macrophage chemotaxis; maintenance of protein location; monocyte chemotaxis; mononuclear cell migration; mRNA processing; negative regulation of apoptosis; negative regulation of endocytosis; negative regulation of extrinsic apoptotic signaling pathway; negative regulation of immunological synapse formation; negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; negative regulation of T cell receptor signaling pathway; neutrophil chemotaxis; positive chemotaxis; positive regulation of angiogenesis; positive regulation of calcium ion import; positive regulation of cell proliferation; positive regulation of dendritic cell differentiation; positive regulation of mononuclear cell migration; positive regulation of serotonin secretion; regulation of extrinsic apoptotic signaling pathway via death domain receptors; regulation of T cell proliferation; RNA splicing; signal transduction; skeletal system development

Research Articles on GAL3

Similar Products

Product Notes

The GAL3 lgals3 (Catalog #AAA2007127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Galectin 3 (GAL3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin 3 (GAL3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAL3 lgals3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-MADGFSLN DA LAGSGNPNPQ GWPGAWGNQP GAGGYPGASY PGAYPGQAPP GGYPGQAPPS AYPGPTGPSA YPGPTAPGAY PGPTAPGAFP GQPGGPGAYP SAPGAYPSAP GAYPATGPFG APTGPLTVPY DMPLPGGVMP RMLITIIGTV KPNANSITLN FKKGNDIAFH FNPRFNENNR RVIVCNTKQD NNWGREERQS AFPFESGKPF KIQVLVEADH FKVAVNDVHL LQYNHRMKNL REISQLGIIG DITLTSASHA MI. It is sometimes possible for the material contained within the vial of "Galectin 3 (GAL3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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